二甲基甲酰胺亚慢性染毒对小鼠神经行为功能的影响

Effects of Sub-chronic Exposure to N,N-dimethylformamide on Neurobehavioral Function of Mice

目的探讨二甲基甲酰胺(N,N-dimethylformamide,DMF)亚慢性染毒对ICR小鼠神经行为功能的影响。方法80只ICR小鼠随机分为4组,分别以1.26、0.63、0.32 g/kg经口灌胃染毒50 d,对照组给予生理盐水(1次/d,50 d)。染毒前和染毒后分别进行Morris水迷宫、避暗箱、穿梭箱和自主活动箱等4项神经行为功能实验。结果高剂量染毒组Morris水迷宫潜伏期较染毒前时间延长,差异有统计学意义(P<0.05);避暗箱实验显示,高剂量组染毒后电击次数增加,潜伏期缩短,与染毒前比较,差异有统计学意义(P<0.05);穿梭箱实验表明,染毒组较对照组电击次数增加,其中,高剂量组与对照组比较,差异有统计学意义(P<0.05);高剂量组主动逃避时间减少,与对照组比较,差异有统计学意义(P<0.01);自主活动实验表明,染毒组较对照组自主活动增多,其中,高剂量组与对照组比较,差异有统计学意义(P<0.05)。结论亚慢性暴露于DMF对小鼠的学习记忆能力有一定的损害作用。

Objective To explore the effects of sub-chronic exposure to DMF on neurobehavioral function in ICR mice. Methods 80 ICR mice were randomly divided into 4 groups. Three experiment groups were instilled with DMF at 1.26, 0.63, 0.32 g/（kg·d） respectively for 50 days and the control group instilled with normal saline once a day for 50 days. Before and after the exposure, behaviors of mice were monitored by Morris water maze, dark box avoiding, shuttle-box, and autonomic activity test. Results The latency of Morris maze in group treated with highest dosage prolonged, the difference was significant（P〈0.05）. The results of dark box avoiding test showed that the number of electric shocks in highest dosage group increased and the latency shortened, the difference was significant （P〈0.05）. The shuttle-box experiments showed that the number of electric shocks in experiment groups became more than that of the control group, and significant difference existed between that of the highest dosage group and that of the control（P〈0.05）. The active escape time in experiment groups decreased. Among them, significant difference existed between that of the highest group and that of the control group（P〈0.01）. Autonomic activity box test showed that autonomic activities in experiment groups became more than that in the control group. The difference was significant when comparing the highest dosage group and the control group （P〈0.05）. Conclusions The results indicate that sub-chronic exposure to DMF might damage in certain extent the learning and memory ability in mice.