摘要
Multilayer film was fabricated on an electrode surface by alternate layer-by-layer(LBL) adsorption of polycationic redox polymer(PEI-Fc) and dye-linked L-proline dehydrogenase(L-proDH).The electrochemistry of the PEI-Fc/L-proDH multilayer modified electrode was investigated by cyclic voltammetry,and the enzyme catalysis mediated by the redox polymer was studied in a solution containing L-proline.It was observed that electron communication between L-proDH and the electrode was achieved with the help of PEI-Fc.A mathematical expression for the current response was evaluated based on the Michaelis-Menten kinetics mode,and the calculated currents fitted well with the experimental data.The kinetic analysis indicates that only a small fraction of the immobilized enzyme was efficiently electrically wired by the redox polymer.
Multilayer film was fabricated on an electrode surface by alternate layer-by-layer（LBL） adsorption of polycationic redox polymer（PEI-Fc） and dye-linked L-proline dehydrogenase（L-proDH）.The electrochemistry of the PEI-Fc/L-proDH multilayer modified electrode was investigated by cyclic voltammetry,and the enzyme catalysis mediated by the redox polymer was studied in a solution containing L-proline.It was observed that electron communication between L-proDH and the electrode was achieved with the help of PEI-Fc.A mathematical expression for the current response was evaluated based on the Michaelis-Menten kinetics mode,and the calculated currents fitted well with the experimental data.The kinetic analysis indicates that only a small fraction of the immobilized enzyme was efficiently electrically wired by the redox polymer.
基金
Supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars of the Ministry of Education,China
the Natural Science Foundation of Tianjin City,China(No.06YFJMJC15000)
