乳腺癌组织中N-乙酰氨基葡萄糖转移酶Ⅴ的活性和临床意义

Activity of N-acetylglucosaminyltransferase Ⅴ in breast cancer tissues and its clinical significance

目的:检测乳腺癌组织中N-乙酰氨基葡萄糖转移酶Ⅴ(GnT-Ⅴ)的活性并分析其临床意义。方法:应用组织芯片技术构建12×10乳腺癌组织阵列块,通过凝集素印迹法检测以白细胞植物凝集素(PHA-L)标记的GnT-Ⅴ在57例乳腺癌和12例乳腺良性病变中的活性,并探讨其与临床病理学参数间的关系。结果:乳腺癌组织中PHA-L阳性89.47%(51/57),胞质阳性,乳腺良性病变组织中PHA-L阳性8.33%(1/12),两者存在显著性差异(P<0.01)。乳腺癌中GnT-Ⅴ活性在不同病理类型、不同淋巴结转移状况、不同病理分期患者中的表达无明显差异。结论:乳腺病灶GnT-Ⅴ高活性提示病变恶性可能大,可以GnT-Ⅴ为靶点,治疗出现复发及转移三阴的乳腺癌患者。

Objective： To explore the activity of N-acetytglucosaminyltransferase V （GnT-V） in breast cancer tissues and to discuss its clinical significance. Methods： The activities of GnT-V were examined in a tissue microarray cohort of 57 breast carcinomas and 12 benign breast lesions. Leetin blotting using Phaseolus vulgaris leukocytic phytohemagglutinin （PHA-L） labeling was used to assay GnT V activity. The relationship between clinicopathological parameters and GnT-V activity was investigated. Results： High activity of GnT-V was observed in 89.47%（51/57） breast cancer tissues, which was significantly higher than that in the benign breast lesions （8.33 %[ 1/12], P〈0.01 ）. No difference in GnT-V activities was observed in breast cancer patients with different pathological types, different lymphatic statuses, and different stages. Conclusion： Higher GnT-V activity in breast cancer tissues indicates a higher risk of malignancy. Targeting GnT- V might be a future way for treatment of triple-negative breast cancer patients with recurrence and metastasis.