COX-2、u-PA和TSP-1在胃癌及周围淋巴结中的表达及意义

Expression of cyclooxygenase-2,urokinase plasminogen activator and thrombospondin-1 in gastric carcinoma and surrounding peripheral lymph nodes

目的:研究COX-2、u-PA和TSP-1在胃癌及周围淋巴结中的表达状况,探讨它们在胃癌浸润和淋巴结转移中的作用和意义。方法:用组织芯片技术和免疫组化法检测COX-2、u-PA和TSP-1的表达。结果:COX-2、u-PA和TSP-1在胃癌组阳性表达率分别为67.7%、78.1%和78.6%,均显著高于对照组(40.0%、6.7%和40.0%,P<0.05)。COX-2表达与浸润深度有关(P<0.05)。u-PA表达与浸润深度和淋巴结转移均有关(P<0.05)。TSP-1表达与浸润深度和淋巴结转移均无关(P>0.05)。COX-2、u-PA和TSP-1在转移淋巴结和对应癌组织间的表达差异无显著性(P>0.05)。COX-2和u-PA、TSP-1的表达呈正相关,u-PA和TSP-1的表达无相关关系。结论:胃癌中COX-2、u-PA和TSP-1高表达;COX-2表达与胃癌的浸润深度有关,u-PA表达与浸润深度和淋巴结转移有关。

Objective：To investigate the expression of cyclooxygenase-2 （COX-2） ,urokinase plasminogen activator（u-PA）, and thrombospondin 1 （TSP- 1） in gastric carcinoma and the surrounding lymph nodes, so as to discuss their roles in the invasion and metastasis of gastric carcinoma. Methods： The expression of COX-2, u-PA, and TSP-1 in the samples was examined by tissue microarray and S-P immunohistochemistry staining. Results： The positive rates of COX-2, u-PA,and TSP-1 in gastric carcinoma were 67. 7%, 78. 1%, and 78. 6%, respectively, which were significantly higher than those in the surrounding lymph nodes （40.0% ,6.7%, and 40.0% ,respectively,P〈0.05）. The expression of COX-2 was significantly correlated with the depth of invasion （P〈0.05）. The expression of u-PA was significantly correlated with lymph node metastasis and depth of invasion （P〈 0.05）. The expression of TSP-1 was neither correlated with lymph node metastasis nor depth of invasion （P〉0.05）. COX-2, u-PA, and TSP 1 expression was not significantly different between metastatic lymph nodes and corresponding gastric carcinoma. COX-2 expression was positively correlated with u-PA,and TSP-1 expression. TSP-1 expression was not correlated with u-PA expression. Conclusion：COX-2, u-PA,and TSP-1 are highly expressed in the gastric carcinoma. COX-2 expression is correlated with depth of invasion. U-PA expression is significantly related with depth of invasion and lymph node metastasis.