贻贝多糖对老龄SD大鼠抗衰老作用

Anti-aging activity of Mytilus coruscus polysaccharide in aged SD rats

目的:研究贻贝多糖对老龄大鼠抗衰老的生物效应。方法:建立24个月龄SD大鼠自然衰老模型,分为老年正常对照组、贻贝粉组(1g/kg)、贻贝多糖低剂量组(200mg/kg)、贻贝多糖高剂量组(400mg/kg),3个月龄大鼠5只为青年对照组,灌胃给药(1次/d);观察大鼠心脏、肝脏、肾、脾的脏器指数,对其心脏、肝脏进行切片观察病理学变化,测定大鼠血清及肝脏中超氧化物歧化酶(SOD)、谷胱甘肽过氧物酶(GSH-Px)、丙二醛(MDA)水平,测定血清主要生化指标。结果:与老年组相比,贻贝多糖高剂量组的心脏、肝脏病理切片H-E染色观察未见明显衰老性改变,其血清、肝脏中SOD、GSH-Px水平增高(P<0.01),MDA含量降低(P<0.01)。结论:贻贝多糖具有抗衰老作用,抗氧化作用可能是其抗衰老作用的机制之一。

Objective：To investigate the anti aging activity of Mytilus coruscus polysaccharide （MP） in aged rats. Methods： We established a 24-month natural aging rat model. Twenty model rats were divided into 4 groups, namely, the aging model group,Mytilus coruscus powder group （MCP 1 g/kg）, high dose MP group （MP 400 mg/kg） ,and low dose MP group （MP 200 mg/kg）. Five three-month-old rats served as young control. The Mytilus coruscus power solutions and MP solutions were given intragastrically on a daily basis for 90 days. The organ indices of the heart,liver, kidney,and spleen of rats were observed; the pathology of the hearts and livers was also observed. The levels of superoxidedismutase （SOD）, glutatio-neperoxidase （GSH- Px） ,and malonaldehyde（MDA） in the serum and liver were determined; and the main serum parameters were also determined. Results：Compared with the aging model group,the heart and liver pathology had no obvious aging changes in the high dose MP group. The serum and liver SOD,GSH-Px levels were significantly higher in the high dose MP group than those in the aging model group （P〈0. 01） ; the MDA level was significantly lower than those in the aging model group （P〈0. 01）. Conclusion： The Mytilus coruscus polysaccharide possess anti-aging activity,and the anitoxidative effect might be one of the mechanism for the anti-aging effect.