摘要
目的:研究贻贝多糖对老龄大鼠抗衰老的生物效应。方法:建立24个月龄SD大鼠自然衰老模型,分为老年正常对照组、贻贝粉组(1g/kg)、贻贝多糖低剂量组(200mg/kg)、贻贝多糖高剂量组(400mg/kg),3个月龄大鼠5只为青年对照组,灌胃给药(1次/d);观察大鼠心脏、肝脏、肾、脾的脏器指数,对其心脏、肝脏进行切片观察病理学变化,测定大鼠血清及肝脏中超氧化物歧化酶(SOD)、谷胱甘肽过氧物酶(GSH-Px)、丙二醛(MDA)水平,测定血清主要生化指标。结果:与老年组相比,贻贝多糖高剂量组的心脏、肝脏病理切片H-E染色观察未见明显衰老性改变,其血清、肝脏中SOD、GSH-Px水平增高(P<0.01),MDA含量降低(P<0.01)。结论:贻贝多糖具有抗衰老作用,抗氧化作用可能是其抗衰老作用的机制之一。
Objective:To investigate the anti aging activity of Mytilus coruscus polysaccharide (MP) in aged rats. Methods: We established a 24-month natural aging rat model. Twenty model rats were divided into 4 groups, namely, the aging model group,Mytilus coruscus powder group (MCP 1 g/kg), high dose MP group (MP 400 mg/kg) ,and low dose MP group (MP 200 mg/kg). Five three-month-old rats served as young control. The Mytilus coruscus power solutions and MP solutions were given intragastrically on a daily basis for 90 days. The organ indices of the heart,liver, kidney,and spleen of rats were observed; the pathology of the hearts and livers was also observed. The levels of superoxidedismutase (SOD), glutatio-neperoxidase (GSH- Px) ,and malonaldehyde(MDA) in the serum and liver were determined; and the main serum parameters were also determined. Results:Compared with the aging model group,the heart and liver pathology had no obvious aging changes in the high dose MP group. The serum and liver SOD,GSH-Px levels were significantly higher in the high dose MP group than those in the aging model group (P〈0. 01) ; the MDA level was significantly lower than those in the aging model group (P〈0. 01). Conclusion: The Mytilus coruscus polysaccharide possess anti-aging activity,and the anitoxidative effect might be one of the mechanism for the anti-aging effect.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2009年第7期786-789,共4页
Academic Journal of Second Military Medical University
关键词
贻贝多糖
衰老
抗氧化剂
Mytilus coruscus polysaccharide
aging
antioxidants