大肠杆菌基因组减小研究进展

Progress in Development of Reduced-Genome Escherichia coli

大肠杆菌是遗传重组领域广泛应用的宿主之一,用于生产重组蛋白、氨基酸和其他化学品。基因组减小可以减少代谢调节网络中的冗余,提高其预测性和可控性。我们介绍了最小基因组的研究策略、应用无痕敲除技术减小大肠杆菌基因组的方法,以及基因组减小后对菌体生长特性、附加体稳定性、重组蛋白表达和代谢的影响。

Escherichia coli is one of the best hosts for genetic recombination and has been used in the pharmaceutical and fermentation industries to make recombinant proteins, amino aicds and other chemicals. Genome size reduction may decrease the redundancy of regulatory and metabolic networks, and provide a more predictable and controllable system than the wild type strain. In this paper we reviewed the research tactics for a minimum genome, the methods of reducing genome by markerless deletion, the growth characteristics of the reduced-genome strain and its effect on the plasmid stability, the recombinant protein expression and the metabolism.