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体外诱导大鼠骨髓间充质干细胞向视网膜色素上皮细胞分化的可行性研究

Feasibility study of differentiation of invitro induced rat bone marrow-derived mesenchymal stem cells into retinal pigment epithelial cells
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摘要 目的探讨体外诱导大鼠骨髓间充质干细胞(rMSCs)向视网膜色素上皮(RPE)细胞分化的可行性。方法采用贴壁筛选法分离、培养Brown—Norway(BN)rMSCs。将反复冻融制成的BN大鼠RPE细胞裂解液加入到rMSCs培养体系中,鉴定被诱导的细胞是否同时表达RPE细胞的特征性标记物细胞角蛋白(CK)与S一100。结果经RPE细胞裂解液诱导的rMSCs生长速度减慢,细胞呈长梭形,周边有毛刺样突起。免疫印迹法和双重免疫荧光标记显示部分经诱导的细胞同时表达CK与S一100。流式细胞术显示14.1%的细胞能够同时表达CK与S-100。结论rMSCs经RPE细胞裂解液诱导后能够向RPE细胞方向分化。 Objective To investigate the feasibility of differentiation of invitro induced rat bone marrow-derived mesenehymal stem cells(rMSCs) into retinal pigment epithelial (RPE) cells. Methods The rMSCs from Brwon-Norway (BN) rats were isolated and cultured by adherent screening method. RPE cells lysate made by repeated freeze-thawing was put into the rMSCs culture system to identify whether the induced cells could express characteristic label cytokeratin(CK)and S-100 simultaneously or not. Results The growth rate of rMSCs induced by RPE cells lysate was slower and protuberant burr surrounded the fusiform cells. The results of irnmunoblotting and double immunofluorescence showed that partial induced cells expressed CK and S 100 simultaneously. The result of flow cytometry indicated that 14.1% induced cells expressed CK and S-100 simultaneously. Conelusion Induced by RPE cells lysate, rMSCs can differentiate into RPE cells.
作者 高斐 董方田
出处 《中华眼底病杂志》 CAS CSCD 北大核心 2009年第4期254-256,共3页 Chinese Journal of Ocular Fundus Diseases
关键词 干细胞/细胞学 骨髓祖代细胞/免疫学 细胞培养技术/方法 细胞分化/免疫学 色素上皮 眼/细胞学 动物 实验 Stem cells/cytology Myeloid progenitor cells/immunology Cell culture techniques/methods Cell differentiation/immunology Pigment Epithelium of eye/cytology Animals, laboratory
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