甲氨蝶呤对寻常性银屑病患者血清金属蛋白酶-9及其组织抑制剂-1的影响研究

Effects of MTX on serum MMP-9 and TIMP-1 in the patients with psoriasis vulgaris

目的:研究基质金属蛋白酶-9(MMP-9)及金属蛋白酶组织抑制剂-1(TIMP-1)在寻常性银屑病发病中的作用,以及甲氨蝶呤(MTX)对它的调节作用,以探讨MTX治疗银屑病的机制。方法:应用双抗体夹心酶联免疫吸附法(ELISA)检测35例寻常性银屑病患者经MTX治疗前、后外周血清中MMP-9及TIMP-1的水平。结果:寻常性银屑病患者血清中MMP-9、TIMP-1、MMP-9/TIMP-1的水平较正常人对照组显著增高(P<0.05)。治疗后血清中MMP-9、TIMP-1、MMP-9/TIMP-1较治疗前显著下降(P<0.05)。结论:MTX可能通过降低血清MMP-9、升高TIMP-1水平而起治疗作用。

Objective： To investigate the regulation of MTX on MMP-9 and TIMP-1 and explore its treatment mechanism in the patients with psoriasis vulgaris. Methods： Serum levels of MMP-9 and TIMP-1 were measured by double antibody sandwich enzyme-linked immunosorhent assay （ELISA） in 35 patients with psoriasis vulgaris before and after treatment with MTX. Results： The serum levels of MMP-9, TIMP-1 and MMP-9/TIMP-1 were significantly higher before treatment than those in after treatment and in normal controls （P〈 0.05）, and there was significant difference between before and after treatment（P〈 0.05）. Conclusions： The MMPs may be very important to the pathogenesis of psoriasis vulgaris. It is indicated that inhibiting the secretion of MMP-9 and increasing the level of TIMP-1 may be involved in the treatment of psoriasis vulgaris with MTX.