摘要
目的:研究基质金属蛋白酶-9(MMP-9)及金属蛋白酶组织抑制剂-1(TIMP-1)在寻常性银屑病发病中的作用,以及甲氨蝶呤(MTX)对它的调节作用,以探讨MTX治疗银屑病的机制。方法:应用双抗体夹心酶联免疫吸附法(ELISA)检测35例寻常性银屑病患者经MTX治疗前、后外周血清中MMP-9及TIMP-1的水平。结果:寻常性银屑病患者血清中MMP-9、TIMP-1、MMP-9/TIMP-1的水平较正常人对照组显著增高(P<0.05)。治疗后血清中MMP-9、TIMP-1、MMP-9/TIMP-1较治疗前显著下降(P<0.05)。结论:MTX可能通过降低血清MMP-9、升高TIMP-1水平而起治疗作用。
Objective: To investigate the regulation of MTX on MMP-9 and TIMP-1 and explore its treatment mechanism in the patients with psoriasis vulgaris. Methods: Serum levels of MMP-9 and TIMP-1 were measured by double antibody sandwich enzyme-linked immunosorhent assay (ELISA) in 35 patients with psoriasis vulgaris before and after treatment with MTX. Results: The serum levels of MMP-9, TIMP-1 and MMP-9/TIMP-1 were significantly higher before treatment than those in after treatment and in normal controls (P〈 0.05), and there was significant difference between before and after treatment(P〈 0.05). Conclusions: The MMPs may be very important to the pathogenesis of psoriasis vulgaris. It is indicated that inhibiting the secretion of MMP-9 and increasing the level of TIMP-1 may be involved in the treatment of psoriasis vulgaris with MTX.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2009年第8期494-496,共3页
Journal of Clinical Dermatology
基金
广东省自然科学基金(07001961)
广州市医药卫生科研基金(2007-YB-140)资助项目