广西扶绥人群GSTM1和GSTT1基因多态性与肝癌易感性关系的研究

Relationship between genetic polymorphisms of glutathione-s-transferase M1,T1 genes and susceptibility to hepatocellular carcinoma in population of Fusui District of Guangxi Zhuang Autonomous Region

目的:探讨GSTM1和GSTT1基因多态性与肝癌易感性关系,以及基因与基因间的相互作用。方法:应用病例-对照分析研究,采用多重PCR技术,对广西扶绥100例肝细胞癌患者、60例正常人群的GSTM1和GSTT1基因型进行检测。将实验结果与临床资料结合进行统计学分析。结果:GSTM1基因空白型在HCC组和正常对照组中的频率分别为59.0%、68.3%(P>0.05);HCC组GSTT1基因缺失频率(33.0%)显著高于正常对照组(18.3%);GSTM1和GSTT1基因同时缺失在肝癌组和对照组中的频率分别为22.0%和3.3%,两者差异有统计学意义。结论:1)GSTM1和GSTT1基因的缺失是通过遗传获得。2)GSTT1基因缺失是HCC的易感因素。3)GSTM1和GSTT1基因联合缺失与HCC的发生呈显著正相关,且两基因具有协同作用,可作为HCC高危人群筛选的有用指标。中华肿瘤防治杂志,2009,16(13)

OBJECTIVE： To explore the relationship between the polymorphisms of glutathione-s-transferase M1（GSTM1） and T1 （GSTT1） and the susceptibility to hepatocellular carcinoma（HCC）, as well as a focus on the gene-gene interaction. METHODS： By case control comparing analysis, the genotypes of GSTM1 and GSTT1 were identified by multiplex polyrnerase chain reaction. All the sam pies （DNA） were detected in 100 patients with HCC, and 60 bealthy controls, coming from Fusui district of Guangxi province. Combining trial results with clinical materials were analysed statistically. RESULTS： The/requenies of GSTM1 null genotype in the HCC group and controls were 59.0% and 68.3% respectively （P〉0. 05）. The frequency of GSTT1 null genotype in the HCC group （33.0%） was significantly higher than that （18. 3%） in the controls. The preva cence of GSTM1 and GSTT1 combined null genotype in HCC was 22.0% as compared with 3. 3% in controls, showing a remarkable difference between them. CONCLUSIONS： 1）Both GSTM1 and GSTT1 null genotypes are acquired through generation. 2）The gene of GSTT1 deficiency is a susceptible factor for HCC development. 3）The gene deficiencies both GSTM1 and GSTT1 are positively correlated with the liver cancer occurrence, and there is a synergistic effect in hepatocarcinogenesis.