EMS1蛋白在喉癌组织中的表达及临床意义

Expression of EMS1 protein and its clinical significance in laryngeal carcinoma

目的:探讨EMS1在喉癌组织中的表达及与各临床病理因素的关系。方法:采用流式细胞术检测40例喉癌组织和40例相应癌旁组织中EMS1的表达,并以20例非喉癌患者喉部正常黏膜组织作对照。结果:喉癌组织中EMS1蛋白的表达量及阳性表达率均明显高于癌旁组织及正常喉黏膜组织(P<0.05),癌旁组织及正常喉黏膜组织间的EMS1表达差异无统计学意义(P>0.05)。EMS1蛋白的表达与喉癌组织中的淋巴结转移、临床分期、分化程度差异有统计学意义(P<0.05);与临床分型、肿瘤大小、吸烟量、年龄和性别差异无统计学意义(P>0.05)。结论:EMS1蛋白的高表达可促进喉癌的发生、发展,其蛋白检测可作为判断喉癌分化、浸润、转移、分期的重要指标。

Objective： To investigate the expression of EMS1 in laryngeal carcinoma and its clinical significance. Method：The expression of EMS1 protein was measured in 40 samples of,40 samples of para carcinoma tissues（which were near to cutting margin of laryngeal carcinoma tissuse over 0.5 cm） ,and 20 samples of normal laryngeal mucosa as controls by Flow Cytometere（Epics XL Ⅱ）. Result：The quantity and percentage of EMS1 protein expression in laryngeal carcinoma tissues was significantly higher than those in para carcinoma and in normal laryngeal mucosa tissues respectively（P〈0.05）. There was no significant expression difference between the para carcinoma tissues and normal laryngeal mucosa tissues. There were positive correlation between the expressions of EMS1 protein and metastasis, pathological grade and clinical stage in laryngeal carcinoma. But there were not relationship with patients＇ clinical classification, tumor size, smoking history, age and sex. Conclusion： The high expression of EMS1 may contribute to the carcinogenesis and development of laryngeal carcinoma. The expression of EMS1 protein is an important index of judging differentiation, infiltration, metastasis and staging of laryngeal carcinoma.