实时荧光定量PCR技术在乳酸菌定量检测中的应用

Application of Real-Time Fluorescent Quantitative PCR in Quantitative Detection of Lactic Acid Bacteria

与传统的菌落计数、杂交技术及PCR技术等相比,实时荧光定量PCR技术作为一种检测和定量微生物的方法,具有较高的灵敏度及可重复性,而且反应过程快速,污染较小。作者综述了实时荧光定量PCR技术的定量原理,荧光检测物质,目的基因序列以及其特点,并且列举了在乳酸菌定量检测中的应用,同时对其应用前景进行了展望。

Real-time fluorescent quantitative PCR （RT-FQ PCR）was a method detecting and quantifying microorganism. Compared with traditional colony count, hybridization and other PCR-technology, RT-FQ PCR has better sensitivity and reproducibility, it was also quicker to perform and has minimized cross contamination. The basic principle of RT-FQ PCR, the chemistries, target gene sequences and characteristic of RT-FQ PCR were described and discussed in this review, furthermore, some applications were presented and the future perspectives were suggested.