三波长-分光光度法测定山楂叶、果总黄酮的含量

Determination of Total Flavonoids in Hawthorn Leaves and Fruits by Three-Wavelength Spectrophotometry

采用三波长-分光光度法测定山楂叶和果中黄酮的含量，消除了由于山楂叶和果中黄酮光谱吸收峰不对称给定量分析造成的影响，并校正了由于干扰组分的吸收光谱具有线性吸收产生的基线倾斜。实验结果：回归方程△A-88．795C～0．2060；相关系数r=0．9994；黄酮质量浓度在0～40．6μg／mL范围内，分别在波长λ1—463nm、λ2—417nm、λ3—382nm测吸光度时，则△A与质量浓度C之间呈良好的线性关系，可按标准曲线法进行定量分析。回收率为96．86％～102．46％；变异系数小于0．136％。方法的准确度与精密度较好，且操作简便易行。

In this study, a three- wavelength spectrophotometry method, could eliminate the absorbance error of interfering components, turbid solution and the scattering effect, was developed to determination of flavonoids in hawthorn leaves and fruits. Back--ground changed with concentration changed and asymmetric absorption peak problems can be solved also. The regression equation of concentration △A was obtained and listed as follows： △A = 88. 795C--0. 2060 with a relation coefficient）, γ= 0. 9994. The experimental results demonstrated the total flavonoids concentrations in 0 - 40.66 /μg/mL with △A obeying linear relation, when the absorbanee was measured at wavelength λ1 =463nm,λ2=417 nm and λa=382 nm. The recovery is 96. 86%- 102.46% and the coefficient of variation is 0. 136%. The method exhibited more advantages over the traditional spectrophotometry method.