大孔树脂纯化蓝莓果中花色苷的研究

Studies on Purification of Anthocyanins from Blueberry Fruits by Macroporous Resins

比较了10种大孔树脂对蓝莓花色苷的吸附和解吸效果,研究了AB-8型大孔树脂对蓝莓花色苷的吸附与解吸条件。结果表明,AB-8型大孔树脂是纯化蓝莓花色苷效果较好的树脂;蓝莓花色苷在AB-8型树脂上的吸附平衡时间为4 h,解吸平衡时间为2 h,吸附的最适质量浓度为750mg/L;30℃,pH 3.0时吸附能力比较强,解吸时宜选用体积分数60%乙醇溶液。该工艺生产的花色苷产品为紫黑色粉末,色价为54.10,回收率为88.20%。

The adsorption and desorption effects of ten kinds of macroporous resins to blueberry anthoeyanins were carefully investigated. Among of them, AB-8 macroporous resins exhibited the excellent characteristics for blueberry anthocyanins purification and then further explored.. It was found that the time of absorption and desorption equilibrium was 4 h and 2 h, respectively, and the concentration of absorption equilibrium was 750 mg/L. At the conditions of pH 3.0 and 30 ℃, the highest adsorption ability for blueberry anthocyanins was achieved, and. 60 % ethanol solution was used to desorpt the anthocyanins blueberry from AB-8 macroporous resins. By combination of those optimum conditions, a purplish dark powder with colour value 54. 10 anthocyanins was achieved with 88. 20% of the recovery rate.