复合维生素B和谷胱甘肽对脂肪肝的保护作用

Protective mechanism of complex vitamin B and GSH on fatty liver

目的研究复合维生素B、还原型谷胱甘肽(GSH)及二者联用对大鼠脂肪肝的保护作用及机制。方法雄性Wistar大鼠随机分为5组:正常对照组、模型对照组、复合B族维生素实验组(T-VB)、谷胱甘肽实验组(T-glu)、GSH与复合VB联用实验组(T-glu+B),每组10只。以CC14、高脂饲料及酒精水建立大鼠脂肪肝模型,实验组分别灌服复合维生素B或(和)GSH,正常对照组和模型对照组灌服生理盐水。连续28天后,测定血脂及肝组织脂质含量、肝组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量等指标,观察肝组织形态学变化。结果与正常对照组相比,其余4组大鼠肝组织出现不同程度的脂肪变性,血清天冬氨酸转移酶(AST)和HDL含量升高,血清及肝组织总胆固醇(TC)、甘油三酯(TG)含量升高,肝组织SOD活性降低及模型对照组血清丙氨酸转移酶(ALT)含量和肝组织MDA含量升高(P<0.05);经实验因素干预后,与模型对照组相比,各实验组大鼠肝脂肪变性明显减轻,血清ALT含量下降,HDL含量明显升高,血清及肝组织TC、TG含量明显降低,肝组织SOD活性升高,MDA含量降低(P<0.05)。结论复合维生素B、GSH对大鼠脂肪肝有一定的保护作用,其作用机制可能为抑制脂代谢紊乱和抗氧化损伤。

Objective To study the protective mechanism of complex vitamin B （Co VB）, GSH and both application on the rat fatty liver. Methods 50 male Wistar rats were allocated to 5 groups randomly, which were normal control group （NC） ,model control group（TC）,intervention of Co VB （T-VB）, intervention of GSH （T-glu）, intervention of Co VB and GSH （T-glu ＋ B）, respectively. Except NC, other groups were treated with a low dose of Carbon tetrachloride （CCl4）, high fat diet and alcohol water solution to produce fatty liver model. At the same time, experimental groups were drenched Co VB or （and） GSH, respectively. NC and TC group was drenched physiological salt solution for 28 days. The serum and liver lipid were quantified. The content of MDA and activity of SOD in liver were measured, and histomorphology changes of the liver were observed, and so on. Results Compared with NC group, rat hepatic cells showed adipose degeneration, and the content of liver MDA and serum ALT was increased in TC group.The content of both serum and hepatic tissue TC, TG were increased, the content of serum AST and HDL were increased, the activity of liver SOD was decreased vs. NC group （ P 〈0. 05）. After intervention, adipose degeneration of experiment groups hepatic cells was obviously inhibited, the content of both serum and （or） hepatic tissue TC, TG, ALT and MDA were decreased （ P 〈 0.05 ）, but the content of serum HDL and the activity of liver SOD were increased vs. TC group（ P 〈 0.05）. Conclusion Co VB and GSH have the protection on fatty liver of rats, the mechanism is that they can inhibit lipid metabolic disorder and antioxidative damage.