饮水硼对大鼠血液成分的影响

Effect of drinking boron on blood composition in rats

目的研究饮水添加不同水平硼对大鼠血液成份的影响。方法192只(28±2)天清洁级SD大鼠,随机分为6组,每组32只,雌雄各半,分别饮用添加硼0、40、80、160、320和640mg/L的蒸馏水,试验期60天。分别于试验的第30天和60天每组随机取鼠16只,雌雄各8只,10%水合氯醛腹腔麻醉,右心房采血,测定红白细胞数、网织红细胞数、血红蛋白含量;数制作血涂片,瑞氏-吉姆萨氏染色,进行白细胞分类计数。结果各试验组的网织红细胞数不同程度升高;硼40、80mg/L组的红细胞数和血红蛋白含量多显著升高(P<0.05),各种白细胞比例变化多不显著;硼160～640mg/L组的红、白细胞数和血红蛋白含量均显著或极显著降低(P<0.05或P<0.01),试验第60天大鼠的中性粒细胞、嗜酸性粒细胞、嗜碱性粒细胞和单核细胞比例多显著降低(P<0.05或P<0.01)。结论饮水添加硼40mg/L对大鼠血细胞的发生和血红蛋白的合成均有明显的促进作用;添加硼80mg/L对大鼠红细胞生成和血红蛋白合成有一定促进作用;添加硼160～640mg/L对大鼠血细胞的发生和血红蛋白的合成均有明显的不良影响,甚至具有明显的毒性作用。

Objective To observe the effects of boron supplemented in water on the blood composition in rats. Methods 192 Sprague-Dawley rats （28 ± 2）d were randomly divided into six groups （ n = 32, 16 male rats and 16 female rats）, and drank the distilled water supplemented with 0, 40, 80, 160, 320 and 640 mg/L boron for 60 days, respectively. At the 30th and the 60th day of experiment period, 16 rats （ n = 8, male = female） from each group were Elected and made into narcosis with 10% Chloral Hydrate. Then the blood were obtained from the right atrium after dissection, the blood smear were made and stained with Wright＇ s and Giemsa＇ s stains at the same time. Then the red cells count, the white cells count, the retieulocyte counts, the hemoglobinometry and the differential leukocytes count were determined. Results In comparison with control group, the reticulocyte percentages of all experiment groups exhibited different degree increases, the RBC numbers and Hb contents in rats consuming 40 and 80 mg/L of boron more significantly raised （P 〈 0.05）, but the changes of the leucocyte percentages were more no significant. The numbers of RBC and WBC and the Hb contents in rats supplemented with from 160 to 640 mg/L of boron all decreased significantly or most significantly （P 〈 0.05 or P 〈 0.01 ）, and the percentages of neutrophil, eosinophil, basophil and monoeytic showed similar changes on the 60th day of experiment time. Conclusion The distilled water supplemented with 40 mg/L of boron could promote the hemopoiesis and Hb synthesis in rats, and the drinking water supplemented with 80 mg/L of boron would have some promotion on the erythropoiesis and Hb synthesis; but when the boron supplementation from 160 to 640 mg/L, the higher doses boron could inhibit the hemopoiesis and Hb synthesis obviously, or even exhibit apparente toxicity action.