粪便潜血检测方法的实用化研究

目的为了寻求一种实际工作中准确实用的便潜血检测方法。方法使用化学法及免疫法对100例患者进行潜血实验检测。结果化学法假阳性率为12%,免疫法假阴性率为9%。结论胶体金单克隆抗体。免疫法特异性、灵敏度高,抗干扰性强,但可出现假阴性结果化学法易出现假阳性结果。在临床工作中两种方法结合使用可获得准确的实验结果,有较好的临床应用价值。

Objective For maximal decreasing the influence in clinical practice from the false negative of the immune method and the false positive of the chemical method, a practical occult blood test in routine tests was investigated.Methods The test of occult blood has been performed in X patients using both immune method and chemical method. Result The false positive rate of chemical method was 6%.Meanwhile,the false negative rate of immune method was 9%.Conclusion Colloidal Gold monoclonal antibody test has a high sensitivity and specificity.At the same time,it was not interfered by many other factors.However, there may be a false negative result by this method. On the other hand, the Pyramidon Chemical method may lead to a false positive result.In clinical test,using immune test associated with chemical test,which have more important value,may get the satisfactory result and identify the bleeding location.