家兔肠缺血-再灌注后各器官损伤变化的实验研究

The changes of different organs after ischemia/reperfusion injury of intestine in rabbits

目的探讨家兔肠缺血-再灌注引起各器官变化的特点。方法选取10只健康家兔,随机分为实验组(5只)与对照组(5只),观察肝、肾、肺、心肌及胃肠的病理改变,检测血浆血浆丙二醛(MDA)、红细胞膜MDA、血浆黄嘌呤氧化酶(XOD)、血浆超氧化物歧化酶(SOD)的变化。结果实验组家兔的肝、肾、肺、心肌及胃肠均有较明显的病理改变。实验组血浆丙氨酸氨基转移酶(ALT)含量、肌酐含量、支气管肺泡灌洗液(BALF)蛋白含量、血浆和红细胞膜MDA及血浆XOD含量明显高于对照组(P<0.05),血浆SOD明显低于对照组(P<0.05)。结论家兔肠缺血-再灌注可导致肝、肾、肺、心肌及胃肠损伤,氧自由基对各器官的破坏可能是病理变化的主要因素。

Objective To investigate the effect of ischemia/reperfusion injury of intestine in rabbits＇ organs. Methods Ten health rabbits were randomly divided into two groups： experimental group （ n = 5 ） and control group（ n = 5 ）. The pathological changes of liver, kidney, lung, myocardium and stomach intestine were observed. The biochemical index including MDA, MDA, XOD, SOD were detected in both groups. Results The obvious pathological changes of liver, kidney, lung, myocardium and stomach intestine were found in experimental group. The contents of ALT, creatinine, BALF protein, MDA, XOD in experimental group were significantly higher than those of control group（ P 〈0.05 ） , however the content of SOD in experimental group was significantly lower than that of control group（ P 〈 0.05 ）. Conclusion The ischemia/reperfusion injury of intestine in rabbits can result in the injury of liver, kidney, lung, myocardium and stomach intestine,which may be mainly due to the damage effect of oxygen free radicals on different organs.