2,4-D对柑橘愈伤组织DNA含量变异及其再生能力的影响

The effect of 2,4-D to DNA content variation and regeneration of Citrus calli

利用不同浓度的2,4-D分别处理柑橘愈伤组织1个月和5个月,再用流式细胞仪检测其DNA含量发生变化的细胞百分率。结果表明:2 mg/L的2,4-D处理柑橘愈伤组织1个月后,显著提高DNA含量发生变化的细胞百分率,暗柳橙的DNA含量发生变化的细胞百分率达到20.5%。随着诱导时间的延长,2,4-D诱导能力降低。利用2 mg/L的2,4-D处理1月后的暗柳橙愈伤组织,转入以蔗糖为碳源的培养基上不能再生胚状体,而转入以甘油为碳源的培养基上能够再生胚状体。本研究为获得柑橘多倍体植株提供更多途径。

Citrus calli were treated with 2,4 - D of different concentration for one month and five months respectively, and their DNA content variation were detected by flow cytometry. The results showed that DNA content variation of Citrus calli treated with 2,4 - D of 2 mg/L for one month was increased evidently, up to 20.5 % in Anliucheng callus. The percentage of DNA content variation decreased when the callus was treated with 2,4 - D for five months. Embryoids can he obtained from Anliucheng callus which were treated with 2,4- D of 2 mg/L for one month on the glycerol culture medium, while did not on the sucrose culture medium. This study provides an alternative way of Citrus breeding of polyploids.