微脉冲半导体激光与氩激光对兔视网膜损伤及修复的组织学研究

Histopathological study on retinal lesions and restoration induced by micropulse diode laser and argon green laser in rabbits

目的观察微脉冲半导体激光与氩激光对兔视网膜损伤和修复的组织学反应。方法在黄斑水肿治疗参数下分别对兔眼行微脉冲阈下光凝和氩激光光凝,观察视网膜组织学改变。结果微脉冲阈下光斑不可见而氩激光可见;激光后两者光镜下视网膜感光细胞损伤不明显但脉络膜小静脉轻度充血,4周后消退;电镜下氩激光光斑处外节排列稀疏,色素上皮细胞(RPE)和节细胞肿胀,外核层细胞异染色质增多,4周后RPE和胶原增生明显,脉络膜内色素颗粒增生;而微脉冲光斑变化不明显。结论微脉冲激光光凝视网膜较氩激光损伤更轻微。

Objective To study the histopathological effect of retinal lesions and restoration induced by micropulse diode laser and argon laser in rabbits. Methods Under the parameter of diabetic macular edema micropulse diode laser and argon laser was used to produce retinal lesions in rabbit eyes. The histopathological alterations were observed. Results The subthreshold spots were undetectable and argon spots were detectable. The photoreceptor cells did not change but there was vascular engorgement in the choroid and it was disappear in 4 week after photocoagulation. The outer membranous discs were arranged disorder and the hydroncus of retinal pigment epithelial （RPE） and nodal cells was observed in argon spots by transmission electron microscope. The heterochromatin became muhy in outer nuclear layer. The hyperplasia of RPE and collagen were obviously in 4 week and the hyperplasia of pigment grains were seen in chorioid. But no changes were seen in micropulse spots. Conclusion The retinal lesions produced by micropulse diode laser were slightly than produced by argon laser.