铁(Ⅲ)-甲磺酸加替沙星荧光体系的研究与应用

Study on the fluorescence system of iron-gatifloxacin mesilate and its application

在pH4．80的NaOAc-HOAc介质中，铁能与甲磺酸加替沙星形成稳定的络舍物，使甲磺酸加替沙星的内源性荧光显著猝灭，据此建立了铁（Ⅲ）-甲磺酸加替沙星的荧光猝灭体系测定铁（Ⅲ）含量的新方法。对影响荧光强度的实验条件进行探讨并优化，该体系的最大激发波长λex=365nm（仪器固定，实为293nm），最大发射波长λem=505nm，铁（Ⅲ）浓度在6．0×10^-8～1．4×10^-5mol／L范围内与荧光猝灭程度成正比，检出限为1．3×10^-8mol／L。该法可直接用于测定水、硅石和金属铝中铁（Ⅲ）含量，相对标准偏差为2．4％～3．2％，回收率为99％～104％，结果与原子吸收光谱法测定结果相一致。

A fluorescent method for the determination of iron（Ⅲ） was developed based on the reaction in that iron （Ⅲ） formed a strong fluorescence complex with gatifloxacin mesilate in the medium of pH 4.80 HOAe-NaOAc buffer solution. The experimental conditions influenced the fluorescence intensity were investigated and optimized. The maximum wavelength of excitation and emission were 365 nm and 505 nm respectively. The linear range was 6.0 × 10^-8 --1.4 × 10^-5 mol/L and the detection limit was 1.3 × 10^-8 mol/L. The method was used for the determination of iron（Ⅲ） in water,silica and metal aluminum samples. The determination results were in good agreement with those of AAS with the recovery of 99%--104% and the relative standard deviation of 2.4%--3.2%.