改性聚乳酸-羟基乙酸/Ⅰ型胶原复合支架的细胞亲和性

Cellular affinity of modified poly-lactic glycolic acid/type-Ⅰ collagen compound scaffold

背景:聚乳酸-羟基乙酸是一种较好的细胞支架材料,但细胞亲和性较差、机械强度不够等缺点又限制了它的应用。因此,聚乳酸类支架材料的改性是非常必要的。目的:研究改性聚乳酸-羟基乙酸/I型胶原复合生物支架的细胞亲和性。设计、时间及地点:体外对比观察实验,于2005-02/12在遵义医学院中心实验室完成。材料:利用多聚赖氨酸对聚乳酸-羟基乙酸改性后与I型胶原构成复合生物支架。方法:将改性聚乳酸-羟基乙酸/Ⅰ型胶原复合支架与聚乳酸-羟基乙酸支架各12块,分别放入2块24孔培养板中,每孔加1×1011L-1第2代兔耳软骨细胞悬液100μL,在37℃、体积分数为5%CO2培养箱中培养4h后,每孔加1mL含血清DMEM培养24h。主要观察指标:①倒置显微镜下观察改性前后支架空间结构。②观察改性后支架吸附细胞的形态结构。③计算改性前后支架对兔耳软骨细胞的吸附率。结果:改性后复合支架的粗糙度增加,其细胞吸附率明显高于聚乳酸-羟基乙酸支架组(P<0.05),细胞形态结构较好。结论:改性聚乳酸-羟基乙酸/I型胶原复合生物支架具有较好的细胞亲和性。

BACKGROUND： Poly-lactic glycolic acid （PLGA） is a good material for cell scaffold, but its poor cellular affinity and mechanical strength limit its application. Therefore, modification of PLGA scaffold is necessary. OBJECTIVE： To explore the cellular affinity of modified PLGA/type [ collagen scaffold. DESIGN, TIME AND SETTING： In vitro comparative observation. The experiment was performed at the Central Laboratory of Zunyi Medical University from February to December 2005. MATERIALS： PLGA scaffold was modified with Poly-L-Lysine and compounded with type- [ collagen. METHODS： Twelve modified PLGA/type ] collagen scaffold and 12 PLGA scaffold were separately placed in 2 24-well culture plates, with 100 pL second passage of rabbit chondrocyte suspension in each well, and incubated in 5% CO2 at 37℃for 4 hours, followed by incubation with 1 mL serum-conditioned DMEM for 24 hours. MAIN OUTCOME MEASURES： Convert phase contrast microscope was used to observe scaffold spatial structure before and after modification; appearance of modified scaffold with attached cells; the attachment rate of chondrocyte on scaffold before and after modification was calculated. RESULTS： Rugosity of the modified PLGA was increased compared with PLGA scaffold, and its cell attachment was significantly better than PLGA scaffold （P 〈 0.05）. Cell appearance was good. CONCLUSION： Modified PLGA/type I collagen scaffold has preferable cellular affinity.