银屑病患者皮损和血清中血管内皮生长因子的表达分析

Expression of Vascular Endothelial Ggrowth Factor in Lesional Skin and Sera From Patients with Psoriasis

目的探讨血管内皮生长因子(VEGF)与银屑病发病的关系。方法①用免疫组化法检测35例银屑病患者皮损、非皮损和10例正常人皮肤中VEGF的表达情况;②用酶联免疫吸附法(ELISA)检测25例寻常性银屑病患者和15例正常人血清中VEGF的表达。结果①VEGF主要表达于银屑病皮损的角质形成细胞、真皮乳头血管内皮及真皮上层部分浸润的炎症细胞内。非皮损部位少量表达,而在正常人表皮中几乎不表达;皮损组有20人以上的患者染色级别在++以上,表达明显高于正常人皮肤,有统计学意义(P<0.05);非皮损组与正常人比较无明显差异,没有统计学意义(P>0.05)。②静止期银屑病患者血清中VEGF表达比正常人高,差异有统计学意义(P<0.05)。结论VEGF可能与银屑病病理过程有关。

Objective To investigate the relationship between vascular endothelial growth factor （VEGF） and the onset of psoriasis. Methods （1）The intensity and site expression of VEGF in 35 psoriatic lesions, non-lesion skin and the skin of 10 normal controls were observed by immuno histochemistory. （2）The serum levels of VEGF in 25 psoriasis vulgaris and 15 normal controls were measured by enzyme-linked immunosorbent assay （ELISA）. Results （1） VEGF were prominently expressed by epidermal keratinocytes and superficial dermal vascular endothelium or perivascular inflammation cells within psoriatic lesions, and rare immanoreactive cells were present within nonlesional skin, whereas almost none expression in normal controls. The expression of psoriatic lesions is much higher than that in normal controls, the dyeing level is more than ＋ ＋ of over 20 patients. It has statistical significance （P〈0.05）. There is no statistical significance （P〉0.05）between non-lesion skin and the skin of normal controls.（2）The serum level of VEGF in stability psoriatic patients is higher than in normal controls. It has statistical significance（P〈O.05）. Conclusion VEGF might play a role in the pathogenesis of psoriasis.