脐血间充质干细胞对脐血单个核细胞体外扩增的支持作用

The supporting capacities of mesenchymal stem cells from human umbilical cord blood in expansion of umbilical cord blood mononuclear cells in vivo

目的探讨脐血源间充质干细胞(mesenchymal stem cells derived from umbilical cord blood,UCB-MSCs)联合外源性细胞因子对脐血单个核细胞(umbilical cord blood mononuclear cells,UCB-MNCs)体外扩增的支持作用。方法从脐血中培养出UCB-MSCs,检测其表面抗原,并以此作为滋养层细胞,将UCB-MNCs接种于无血清培养体系中培养10d,检测有核细胞总数(MNCs)、CD34+、CD133+细胞数、集落形成单位数(CFU)和(G2+M+S)期细胞含量的变化。结果从脐血分离、培养出UCB-MSCs,稳定表达CD29、CD105和CD44,不表达CD34和CD133。外源性细胞因子及UCB-MSCs均支持UCB-MNCs的扩增,但以细胞因子联合UCB-MSCs组效果最好(P<0.05)。结论从脐血中能成功分离培养出间充质干细胞,并完成细胞表型的初步鉴定。外源性细胞因子联合UCB-MSCs可有效扩增UCB-MNCs。

Objective To explore the role of mesenchymal stem cells derived from human umbilical cord blood （UCB- MSCs） in combination with exogenous eytokines in supporting the aplification of umbilical cord blood mononuclear cells （UCB-MNCs）ex vivo. Methods MSCs acted as trophoblastic ceils were obtained from umbilical cord blood,then the cellular surface antigens was detected. UCB-MNCs cultured in a serum-free system. On the day 10,total MNCs were counted,the proportion of CD34^＋, CD133^＋ ceils and cellular cycle （G2＋M＋S） were quantitated,also hematopoietie progenitor cells were assessed by semisolid culture assay. Results MSCs isolated and cultured from umbilical cord blood were uniformly negative for CD34,CD133,but positive for CD29,CD105 and CD44. Compared with other greups,on day 10 the amplification of total MNCs,the proportion of CD34^＋ ceils,CD133^＋ cells,cellular cycle （stage G2＋M＋S） nnd colony forming unit（CFU） were higher in the group both supported with UCB-MSCs and cytokines（P〈0.05）. Conclusion MSCs has been isolated and cultured from human umbilical cord blood preliminary phenotype identification has also been accomplished. UCB-MSCs in combination with exogenous cytokines can efficiently support the amllification of UCB-MNCs ex vivo.