流式细胞术分析不同分期的恶性肿瘤外周血T淋巴细胞亚群的变化

The change of the immunocyte in peripheral blood of the patients with cancer in different stage by FCM

目的:研究肿瘤患者不同分期的外周血T淋巴细胞亚群的变化。方法:采用流式细胞术对36例肿瘤患者外周血T细胞及其亚群进行检测,并对不同分期患者的结果进行分析比较。结果:36例肿瘤患者CD3+T、CD+4T细胞水平均下降,Ⅳ期患者的CD+3T细胞水平与Ⅰ、Ⅱ期比较有显著性差异(P<0.05);Ⅳ期患者的CD+4T细胞水平与Ⅰ、Ⅱ期比较有非常显著性差异(P<0.01),与Ⅲ期比较亦有显著性差异(P<0.05);CD+8T细胞则随临床分期的提高略有升高,但无统计学差异;CD4+/CD8+的比值随临床分期的提高而降低,Ⅳ期患者与Ⅰ、Ⅱ期比较差异显著(P<0.05);NK细胞随临床分期提高呈现轻度降低后增加的趋势,但无统计学差异。结论:恶性肿瘤患者随着TNM分期的提高,其机体的细胞免疫功能呈现逐渐下降的趋势。

Objective：To investigate immune function of the cells in the peripheral blood who suffer from cancer in different stage. Methods：Using FCM to analyse the change of the immunocyte in peripheral blood of the 36 patients with cancer in different stage. Results：The CD：（ T cells were significantly lower in the 4th stage than these in the first or second stage（ P 〈 0. 05 ） ;CD4 T cells were significantly lower in the 4th stage than these in the first or second stage（P 〈 0. 01 ） , also significantly lower than the patients in the 3th stage（P 〈 0.05） ;CD8^＋ T cells were upper a little whit the patients＇ stage grow, but no significantly different; CD4＋/CD8＋ was signifieantly lower with the patients＇ stage grow, were significantly lower in the 4th stage than these in the first or second stage（P 〈 0. 05 ; The change of the NK ceils had no regulation in different stage. Conclusion： It shows that the patients＇ immune function of the cells should be lower with the patients＇ stage of TNM grow.