烟雾吸入性损伤修复模型IL-1β和PGE2的动态变化分析

Analysis of dynamic changes of interleukin 1β and prostaglandin E2 in smoke inhalation injury repair model

目的:探讨IL-1β和PGE2在新西兰白兔烟雾吸入性损伤修复中动态水平变化.方法:新西兰白兔36只,随机分为正常对照组、烟雾吸入性损伤后第1,4,7,14,21日,共6组,应用ELISA方法检测各组IL-1β和PGE2的水平.建立烟雾吸入性损伤修复模型,光镜下观察烟雾吸入对新西兰白兔气管、肺组织的病理变化.结果:①病理变化表明烟雾吸入性损伤第1,4,7日组以损伤后炎症反应为主(损伤期),第14,21日组以损伤后修复为主(修复期);②ELISA结果表明:IL-1β含量在烟雾吸入性损伤后第1日[(196.2±67.8)ng/L]立即升高,与正常对照组[(7.1±0.2)ng/L]比较有统计学差异(P<0.05),至第7日[(398.5±161.4)ng/L]达到顶峰后逐渐下降,至第21日[(9.3±3.7)ng/L]最低,与正常对照组比较无统计学差异;PGE2含量在烟雾吸入性损伤后第1日[(4.4±1.0)μg/L]至14日[(120.9±61.1)μg/L]逐渐升高,在第14日达到顶峰后下降,第14日组与正常对照组[(4.4±1.0)μg/L]比较有显著差异(P<0.05),至第21日[(6.4±2.8)ng/L]最低,与正常对照组比较无统计学差异.结论:通过建立烟雾吸入性损伤修复模型,观察IL-1β与PGE2的动态水平变化,可为临床上判断烟雾吸入性损伤修复过程不同阶段(损伤期、修复期)提供帮助,也表明IL-1β与PGE2在烟雾吸入性损伤修复过程中可能具有重要作用.

AIM：To investigate the dynamic changes of interleukin 1β （ IL-1β ） and prostaglandin E2 （ PGE2 ） levels in smoke inhalation injury repair in New Zealand white rabbits. METHODS ： New Zealand white rabbits （ n = 36 ） were randomly divided into normal control group, and 1,4, 7, 14 and 21 d after smoke inhalation injury groups, with 6 animals in each group. ELISA was used to measure the levels of IL-1β and PGE2. The smoke inhalation injury repair model was established and the pathologic changes of smoke inhalation on trachea and lung tissues were observed by light microscope. RESULTS： The pathologic changes showed that mainly inflammatory reaction （injury stage） was seen in 1, 4 and 7 d after injury groups and mainly repair （repair stage） was observed in 14 and 21 d after injury groups. The results of ELISA showed that IL-1β concentration was upregn- lated in 1 d group[ （ 196.2 -±67.8） ng/L1 after smoke inhalation injury, higher than that in normal group[ （7.1±0.2） ng/L, P 〈 0.05 ]. IL-1β concentration peaked at day 7 [ （ 398.5 ± 161.4 ） ng/L] and then began to decrease. IL-1β concentration was the lowest at day 21 [ （9.3 ± 3.7） ng/L] and no significant difference was observed compared with that in normal group （P 〉 0.05 ）. PGE2 concentrations were upregnlated gradually from 1 d [ （4.4 ± 1.0）μg/L] to 14 d[ （ 120.9 ± 61.1 ） μg/L] following smoke inhalation injury. PGE2 concentrations peaked at day 14 and then began to decrease, with significant difference from those in normal control group [ （4350 ± 1021 ） ng/L,P 〈 0.05 ]. PGE2 concentration was the lowest at day 21 [ （6423± 2812 ） ng/L], with no significant difference from that in normal group （P 〉 0.05）. CONCLUSION： The dynamic changes of IL-1β and PGE2 levels in smoke inhalation injury repair may indicate the different stages （injury stage or repair stage） of smoke inhalation injury repair process. IL-1β and PGE2 levels may important role in the smoke inhalation injury repair.