摘要
目的:探讨原癌基因Pokemon(POK erythroid myeloid ontogenic)在大肠癌组织中的表达及其与临床特征之间的关系.方法:采用逆转录酶聚合酶链反应(RT-PCR)方法检测47例大肠癌患者癌、癌旁组织、癌以远大肠组织中Pokemon mRNA的表达水平,分析其与临床特征之间的关系;采用免疫组化ABC法检测53例大肠癌组织中Pokemon蛋白的表达,结合随访资料分析其与患者生存时间的关系.结果:大肠癌癌组织和癌旁组织中Pokemon mRNA水平显著高于正常大肠组织(P<0.05),而癌与癌旁组织中的表达无显著性差异;Pokemon mRNA的表达水平与大肠癌Dukes分期、分化程度及淋巴结转移相关(P<0.05),而与患者的性别、年龄、吸烟、饮酒、肿瘤位置、大小及浸润深度无关;Pokemon表达阴性的大肠癌患者存活率明显高于阳性表达患者(P<0.05).结论:Poke-mon在大肠癌和癌旁组织中高表达,并对大肠癌的预后评价有重要意义,且可能成为大肠癌治疗中有效的新的靶基因.
AIM： To investigate the expression and significance of Pokemon（ POK erythroid myeloid ontogenic ） proto-oncogene in eolorectal cancer. METHODS： The expression of Pokemon in the tumor tissues, corresponding tumor adjacent tissues and the surrounding tissues collected from 47 operation-treated cases of colorectal cancers was detected by reverse transcription-polymerase chain reaction（ RT-PCR）, and the clinical and histopathologic records were retrospectively analyzed. The expression of Pokemon protein was detected by immunohistochemical（IHC） techniques in 53 cases of colorectal cancer to explore its relation with the survival time of the patients. RESULTS ： The expression of Pokemon mRNA in eolorectal tumor and corresponding tumor adjacent tissues, with no significant difference between them（P =0. 054） , was markedly higher than that in the surrounding tissues （ P 〈 0.05 ）. It was correlated with Dukes staging, tumor differentiation degree and lymph node metastasis condition（P 〈0.05 ）, but was not associated with the patients＇ gender, age, smoking and drinking, and tumor position, size and infiltration depth （ P 〉 0.05 ）. The survival rate of patients with negative Pokemon expression was significantly higher than that of patients with positive Pokemon expression（P = 0. 035 ）. CONCLUSION： Pokemon is overexpressed in eolorectal cancer, which may have some clinical significance in prognostic evaluation and offer an effective new target for treatment of colorectal cancers.
出处
《第四军医大学学报》
北大核心
2009年第15期1387-1390,共4页
Journal of the Fourth Military Medical University
