流动注射化学发光法测定维生素B_6

Determination of Vitamin B_6 by Flow Injection Chemiluminescence Method

基于维生素B6对鲁米诺一过氧化氢化学发光反应的增敏作用，结合流动注射技术，建立了流动注射化学发光反应测定维生素B6的新方法。该方法的线性范围为5．0×10^-8～2．0×10^-6g/mL，检出限为2．3×10^-8g/mL。对5．0×10-6g/mL的维生素B6进行11次平行测定，相对标准偏差为2．8％。实验表明该方法简单、快速、灵敏。

A new flow- injection chemiluminescence method for the determination of Vitamin B6 was developed. It is based on the catalysis of Vitamin B6 to chemilumincecrce reaction between luminol and hydrogen peroxide. The linear range extends from 5.0× 10^-8 to 2.0×10^-6 g/mL and the detection limit is 1.3 × 10^-8 g/mL. The relative standard deviation （RSD） is 2.4% for 5.0 × 10^-6 g/mL Vitamin B6 solution in eleven repeated measurements. The method has the advantages of simplicity, rapidity and sensitivity.