摘要
目的建立一种常规高效液相色谱法测定依托泊苷植入剂的有关物质及含量。方法采用C18柱,乙腈-醋酸盐缓冲液(pH4.0)(2∶5)(取醋酸钠5.44 g,加水溶解并稀释至2000 ml,用冰醋酸调节pH值至4.0)为流动相,检测波长:230 nm。降解产物制备方法为将溶液置80℃水浴中破坏30 min。结果该法能很好地分离依托泊苷及有关物质,检测限为0.05μg。结论本方法同时检测依托泊苷植入剂的有关物质及含量,方法准确、方便、实用性强。
Aim To establish an HPLC method for the determination of etoposide implants and related substances. Methods C18 column was adopted. The mobile phase was composed of acetonitrile-acetate buffer solution （2：5 ） ,with 5.44 g of sodium acetate dissolved in 2000 ml of water,adjusted with glacial acetic acid to a pH of 4.0. The detection was carried out with a UV detector at 230nm. The preparative method of the degradation product was to put the solution in the water-bath with a temperature of 80℃ for 30 min. Result The method could separate etoposide from the relative substances. The detection limit was 0.05 μg. Conclusion The method is good and accurate.
出处
《安徽医药》
CAS
2009年第7期751-752,共2页
Anhui Medical and Pharmaceutical Journal
