132例乙型肝炎病毒感染者基本核心启动子变异分析

Mutation of hepatitis B virus basic core promoter in 132 cases of HBV infected patients

目的研究乙型肝炎病毒（HBV）感染者基本核心启动子（BCP）变异情况。方法收集HBV感染者血清标本132份（HBVDNA均阳性），用半巢式聚合酶链反应扩增HBVC基因部分片段，产物纯化后直接测序，检测BCPT1762／A1764变异。用S基因错配聚合酶链反应（PCR-RFLP）法确定HBV基因型。结果51例乙型肝炎e抗原（HBeAg）阳性患者BCPT1762／A1764双变异检出率为27．45％，81例HBeAg阴性患者BCPT1762／A1764双变异检出率为62．96％，两组比较，差异有高度显著性（χ^2=15．79，P=0．00）。BCPT1762／A1764双变异的HBV感染者B基因型检出率为33．85％，明显低于C基因型的检出率66．15％（χ^2=24．25，P=0．00）。结论HBV感染者普遍存在BCPT1762／A1764双变异，以C基因型感染者多见。

Objective To study the hepatitis B virus basic core promoter （BCP） mutation in HBV infected patients. Methods The serum samples from 132 HBV infected patients （all were HBV DNA positive） were collected. The gene covered HBV BCP were amplified by nested polymerase chain reaction （nPCR）. The PCR products were sequenced directly, and the mutations in BCP T1762/A1764 were determined by sequence analysis. HBV genotypes were also detected by restriction fragment length polymorphism based on S gene PCR products. Results The mutation rates of BCP T1762/A1764 were 27. 45% in 51 cases of HBV infected patients with HBeAg（ ＋ ）,62. 96% in 81 cases of HBV infected patients with HBeAg（ - ）. The mutation of patients with HBeAg（ - ）was significantly higher than those with HBeAg（ ＋ ）（χ^2= 15. 79, P = 0. 00）. The detection rate of B gene mutation of BCP T1762/ A1764 in HBV infected patients was 33. 85%, which was obviously lower than 66. 15% of C gene mutation （χ^2= 24. 25, P = 0. 00）. Conclusion The mutation in BCP T1762/A1764 generally exist in HBV infected patients and common in genotype C.