人重组促红细胞生成素增强耐缺氧作用的实验研究

Study on enhancing anoxia endurance function of recombinant human erythropoietin in mice

目的观察人重组促红细胞生成素(EPO)对小鼠缺氧耐受力的影响。方法小鼠随机分为EPO组和对照组,经皮下注射不同剂量的EPO和0.9%氯化钠注射溶液,1次/d,连续1周。检测小鼠血红蛋白、红细胞比容改变,测定常压耐氧实验、亚硝酸中毒存活试验以及急性脑缺血性缺氧试验等指标。结果EPO能明显增加小鼠血液内血红蛋白含量和红细胞比容(与对照组相比,P<0.01),缺氧存活时间显著延长(与对照组相比,P<0.01)。结论EPO能明显增强机体的耐缺氧能力。

Objective To observe the effects of EPO on hypoxia tolerance in mice. Methods The mice were randomly divided into EPO-treated group and control group, the mice were subcutaneous injection administered different doses of EPO or normal saline respectively once a day for 7 days. Then, the levels of hemoglobin and hematocrit were determined. The survival times of acute hypoxia were measured in anoxia with normal air pressure,in poisoning by nitrite and in acute lack of blood in brain. Results The levels of hemoglobin and hematocrit in EPO group were markedly increased,compared with those of control group,P 〈 0.01, and the survival times of hypoxia in mice were significantly prolonged, compared with control group, P 〈 0.01. Conclusion EPO has a remarkable effect in enhancing the tolerance of hypoxia in the mice.