摘要
卵黄蛋白原(Vitellogenin,Vtg)是卵黄蛋白(Vitellins,Vn)的前体.无脊椎动物的Vtg主要在肝胰腺、卵巢或脂肪体合成,脊椎动物主要在肝脏合成.在总结国内外相关研究基础上,对基于环境雌激素评估的Vtg研究进展进行了综述.目前的研究已经证明,Vtg的N末端氨基酸序列在动物进化中既具有高度的保守性,又具有一定的特异性.Vtg作为检测环境雌激素效应的生物标志物之一,在环境雌激素类化合物污染的评价中被广泛采用.Vtg的检测方法包括蛋白直接定量检测和mRNA定量分析,选择检测技术要依据实验设计,对于长时间的暴露实验,可通过检测蛋白来完成,短时间的暴露实验则适合半定量RT-PCR检测VtgmRNA的表达.在Vtg的检测中应考虑动物种属的特异性.
Vitellogenin （Vtg）is the precursor of vitellins （Vn）in oviparous animals. Vtg is synthesized in hepatopancreas, ovary or fat body in invertebrates, whereas it is synthesized in liver of vertebrates. Recent progress on Vtg based on estimating environmental estrogen was reviewed. It was demonstrated that N-termianal amino acid sequences of Vtg had high conservation and definite specificity at present. Vtg is extensively used to assess the compounds of environmental estrogen, which acts as one of biomarkers of detecting environmental estrogens. The specificity of Vtg in different animals is various. The methods of detecting Vtg are varied, including the direct quantitative detection of protein and quantitative analysis of mRNA. Examine technique is selected according to its experiment design. The detection Vtg might be sufficient to a long term exposed studies. However, detection Vtg mRNA with semi-quantitative RT-PCR may be favoured for a experiment exposed with short time.
出处
《生态毒理学报》
CAS
CSCD
2009年第3期332-337,共6页
Asian Journal of Ecotoxicology
基金
国家自然科学基金项目(No.30770243)
