中心复合设计射线法考察离子液体C_(16)H_(31)ClN_2与乐果的毒性相互作用

Study on the Toxicity Interaction between Dimethoate and C_(16)H_(31)ClN_2 by Central Composite Design with the Fixed Concentration Ratio Ray

离子液体(ILs)是一种用于替代传统易挥发有机溶剂的新型"绿色"溶剂.由于不挥发、不会对大气产生污染而得到广泛应用.但是某些ILs易溶于水,其自身毒性能够对生态环境造成潜在影响,这已引起诸多学者对ILs毒性的研究兴趣.然而ILs与其它污染物的毒性相互作用目前研究很少.论文选取咪唑类离子液体C16H31ClN(2DMI)与有机磷杀虫剂乐果(DIM)作为目标化合物,以青海弧菌Q67为检测生物,采用微板毒性分析法测定了目标化合物及其混合物的毒性.为全面考察不同浓度范围DMI与DIM的毒性相互作用,将中心复合设计与固定浓度比射线法有机结合起来构建5个不同浓度比的混合物射线,通过浓度加和与独立作用模型对混合物射线进行比较评估.结果表明在DMI浓度较大且DIM浓度较低时,DMI与DIM之间存在明显拮抗作用,而在其它浓度范围内两者之间为加和作用.

Ionic liquids （ILs）are a kind of novel green solvents designed to replace traditional volatile organic solvents. Due to their non-volatility, ILs have no such trend to pollute the air and they are widely used in industries. However, some ILs can dissolve in water, and they still have potential ecotoxicities. The toxicities of ILs themselves to organism have been reported in the literatures. However, the toxicity interaction between ILs and other pollutants coexisting in aquatic ecosystems has been poorly studied. An imidazole-type IL, C16H31ClN2 （DMI）, and a pesticide, dimethoate（DIM ） were, therefore, selected as target compounds, and their individual toxicity and mixture toxicity were determined by the microplate toxicity analysis using Vibrio qinghaiensis sp.-Q67 as the indicator organism. To systemically examine the toxicity interaction between DMI and DIM with the different concentration compositions, five mixture rays were designed by a simplified central composite with the fixed concentration ratio ray （CCDray）. The concentration addition and independent action models were used to predict and evaluate the significant antagonism between high concentration DMI and low concentration compositions. toxicity interaction. Results showed that there was a concentration DIM while they are additive in other