三唑磷降解菌的筛选及降解特性研究

Screening of Triazophos Degrading Bacteria and Study on Its Degradation Characteristics

[目的]为三唑磷的微生物降解提供参考。[方法]通过初筛和复筛试验,从某农药厂污水中分离得到1株降解三唑磷的菌株TF413,用B iolog微生物自动分析系统对该菌株进行鉴定,并研究培养基组成、初始pH值、培养温度、装液量、接种量等对菌株降解三唑磷特性的影响。[结果]经鉴定,TF413菌株为粪产碱杆菌(Alcaligenes faecalis),其降解三唑磷的最适温度为32℃、最适初始pH值为7.0,最适装液量为100 m l三角瓶中装50 m l培养基,最佳培养基为营养肉汤,接种量对TF413降解三唑磷的效果无明显影响。[结论]TF413以共代谢的方式降解三唑磷,培养72 h对三唑磷的降解率为70.83%。

[ Objective ] The study was to provide the reference for biodegradation of triazophos. [ Method ] Trough primary screening and secondary screening tests, a strain of triazophos degrading bacteria TF413 was obtained from the sewage of one insecticide factory. The strain was identified by Biolog microbial identification system, and the effects of medium composition, primary pH value, culture temperature, liquid vol- ume in flask, inoculation amount on degradation characteristics of TF413 on triazophos were studied. [ Result] According to the result of iden- tification, TF413 belonged to Alcaligenesfaecalis. The optimum temperature for it degrading triazophos was 32 ~C, and !he optimum primary pH value was 7. O, the optimum liquid volume in flask was 50 ml medium in 100 ml erlenmeyer flask, the optimum medium was nutrient broth, the inoculation amount had no obvious effect on degradation effect of TF413 on triazophos. [ Conclusion] TF413 degraded triazophos in method of cometabolism, and the degradation rate of TF413 on triazophos was 70.83% after 72 h of culture.