花外周血细胞显微结构

Microscopic Structure of Peripheral Blood Cell of Hemibarbus maculatus

[目的]描述花[鱼骨]各类血细胞，为鱼类血液学、比较细胞学积累基础资料，并为花鲭人工养殖、疾病诊断等工作提供参考。[方法]对28尾花[鱼骨]尾静脉取血，常规方法每尾制作血涂片2～5张，Gimesa和wrjght染液联合染色，Nikon—DXM1200数码显微摄影系统观察和拍照，ImageProplus软件随机测量各类血细胞。[结果]试验得出，红细胞有直接分裂现象，成红细胞有两种类型，Ⅰ型和网织红细胞关系密切；嗜中性粒细胞一般呈球形，核形多变，单个核居多，偶见二分叶核，偶见衰老解体者；嗜酸性粒细胞一般呈球形，胞核与嗜中性粒细胞胞核情况极为相似；单核细胞具伪足样突起，核较大，约占整个细胞1／2左右，较大者可达3／4；淋巴细胞呈不规则圆形，非定量地分为“大”“小”两类：血栓细胞形态多样，个别类型难以归类，有直接分裂现象；未发现嗜碱性粒细胞。[结论]花[鱼骨]外周血中有6种血细胞，无嗜碱性粒细胞。

[ Objective ] All kinds of blood cells of Hemibarbus maculatus were described in order to collect the material of its hematology and compared cytology and provide the reference for its artificial raising and disease diagnosis. [ Method ] The blood of 28 Hemibarbus maculatus fishes were made and stained with Wright and Giemsa＇s. The morphological characteristics of tbe blood cells were evaluated with the Nikon- DXMI200 digital microphotography and tested with Image Pro plus. [ Results] The result indicated that the phenomenon of red blood cell was direct cell division. There were two types of red blood cells, Type I was close related to the reticulocyte. The neutrophilic granulocyte was generally spherical form, with single nucleus with muhiply variation and few in two-lohopodia nucleus and the dissolution of aging cell. The eosinophilic granulocyte was generally spherical form with same character as neutrophilic granulocyte. The monocyte was with the pseudopodialike protrusions, large nuclear occupied about 1/2 of whole cell, some 3/4 of whole cell. The lymphocytes was irregular spherical form and two classes of lymphocytes（ large and small） were seen, but no quantitative distinction was made between these. Thrombocyte shape was variable being either elongate, oval, spiked, spindle or some unable-describe forms. The basophils were absent. [ Conclusion] There were 6 types of peripheral blood cells of Hemibarbus maculates and not basophils.