不同浓度牛磺酸对兔角膜内皮细胞的影响

Effects of different concentrations of taurine on rabbit corneal endothelial cells

目的探讨牛磺酸对兔眼角膜内皮细胞的毒副作用。方法取家兔6只(共12眼),每只眼制取6片组织片,用组织块培养法培养兔角膜内皮细胞,细胞接种于12孔培养板,分别加入2%、4%、6%、8%、10%的牛磺酸液(同一动物的左右眼加同一浓度牛磺酸溶液),设立空白对照。倒置显微镜观察角膜内皮细胞的生长情况;在培养的第1、2、4、6、8天,将部分培养细胞用Wright染色后观察细胞形态。结果空白对照和加入2%、4%、6%牛磺酸溶液培养的角膜内皮细胞,1周后均形成内皮细胞层,细胞形态呈六角形或类圆形。加入8%牛磺酸液培养的角膜内皮细胞,在第4天时生长不良,细胞胞体小;第8天时培养细胞死亡。加入10%牛磺酸液培养的角膜内皮细胞,在第2天时出现生长不良,细胞胞体小,部分细胞已死亡。同一动物的左右眼角膜内皮细胞生长速度和细胞形态相似。结论2%～6%牛磺酸对兔角膜内皮细胞的生长无不良影响,提示该浓度范围的牛磺酸对角膜内皮细胞无毒副作用。

Objective To investigate the adverse effects of taurine on rabbit corneal endothelial cells. Methods Six rabbits （ 12 eyes） were selected, and 6 histologic sections were prepared from each of the eyes. Rabbit corneal endothelial cells were cultured by explant culture method. Ceils were innoculated on a 12-well tissue culture plate, 2%, 4%, 6%, 8% and 10% taurine solutions were added respectively （ cells from the right and left eyes of the same rabbit were added the same concentration of taurine solution）, and blank control was established. The growth of corneal endothelial cells was observed by inverted microscopy, and cell morphology on the 1^st , 2^st , 4^th, 6^th and 8^th day of culture was observed with Wright staining. Results Corneal endothelial cells cultured with 2%, 4% and 6% taurine solutions and those of blank control formed endothelial cell layers after culture for one week, and the cells exhibited hexagonal or round-like morphology. Corneal endothelial cells cultured with 8% taurine solution appeared to be undergrowth with small cell body on the 4^th day, and cell death occurred on the 8^th day. Corneal endothelial cells cultured with 10% taurine solution turned out to be undergrowth with small cell body on the 2^nd day, and cell death had occurred. The same growth velocity and cell morphology were observed in the corneal endothelial cells from the right and left eyes of the same rabbit. Conclusion Taurine with concentration between 2% and 6% has no adverse effects on the growth of rabbit corneal endothelial cells.