活血跌打膏的提取工艺研究

Study on Extractive Technology of Huoxue-Dieda Paste

目的:优选活血跌打膏的提取工艺。方法:采用正交分析法,测定挥发油含量,大黄素和绿原酸含量。结果:挥发油提取时,加水量(A)、蒸馏时间(C)二因素均对挥发油含量有显著性影响(P<0.05,P<0.01)。乙醇回流提取时,乙醇用量(D)对大黄素含量有显著性影响(P<0.01),乙醇浓度(A)和提取次数(C)对绿原酸含量有显著性影响(P<0.01,P<0.01)。结论:本方最佳提取工艺为挥发性药材先用8倍量水浸泡6h,水蒸汽蒸馏法提取挥发油8h,其药渣与其余药材一起用10倍量65%乙醇回流提取3次,每次1h。

To optimize the extractive technology for Huoxue-Dieda paste. Methods： Orthogonal experiment was used to determine the contents of volatile oil, emodin; and caffeotannic acid. Results： At the time of volatile oil abstracted, the quantity of adding water, distillation time had distinct effects to the content of volatile oil（P〈0.05,P〈0.01）; at the time of recirculation of ethanol extract, so did ethanol dosage（P〈0.01）, the concentration of ethanol and extractive times had distinct effects to the content of caffeotannic acid（P〈0.01） .Conclusion： The best extractive technology is that volatility drugs were soaked 6 hours with 8 times water, water vapor distillation extracted volatile oil for 8 hours, gruffs and other drugs were extracted 3 times by the recirculation of 10 times of 65% ethanol, 1 hour every time.