摘要
研究了巴戟天多糖(polysaccharide from Morinda officinalis How,MOP)在离子交换填料DEAE Sepharose CL-6B上的吸附行为,通过静态吸附实验考察了缓冲液的pH值和离子强度对平衡吸附量、吸附等温线和吸附动力学的影响。结果表明,在实验浓度范围内,吸附平衡数据符合单分子层吸附的Langmuir方程,通过拟合数据可直观地反映pH值和离子强度对MOP吸附的影响;MOP在DEAE-Sepharose CL-6B上的吸附随着缓冲液pH值的上升而增加,低离子强度的缓冲液有利于多糖吸附。
The adsorption properties of polysaccharides from Morinda officinalis （MOP） onto an ion exchange adsorbent, DEAE Sepharose CL-6B, were studied. The effects of pH and ion strength on equilibrium adsorption capacity, isotherm and dynamic curve were discussed by static adsorption experiments. The equilibrium adsorption data obtained could be described well by the Langmuir isotherm equation with high correlation coefficients, suggesting the presence of monomolecular adsorption. The fitted curves could visually demonstrate the influences of pH and ion strength on MOP adsorption onto DEAE Sepharose CL-6B. This adsorbent exhibited better adsorption performance to MOP under higher pH and lower ion strength of buffer solution.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第15期109-113,共5页
Food Science
基金
广东省科技攻关项目(2003C104025)
关键词
巴戟天
多糖
离子交换填料
吸附
Morinda officinalis
polysaccharides
ion exchange
adsorption
