黄花菜提取物对亚硝化反应的抑制能力研究

Suppression of Nitrosation by Extracts of Daylily (Hemerocallis fulva) Flowers

对黄花菜抑制亚硝化反应的能力及其影响因素进行研究。采用比色法测定黄花菜提取物的亚硝酸盐清除率和亚硝胺抑制率。结果表明,黄花菜抑制亚硝化反应功能成分的提取方法为:70%甲醇为提取试剂,超声波辅助提取0.5h,在该条件下,黄花菜提取物的亚硝酸盐清除率和亚硝胺抑制率分别为66.9%和54.7%。在冷藏过程中,黄花菜的亚硝酸盐清除能力和亚硝胺抑制能力变化不大;四个品种中(孟子花、白花、细叶和倒杆),倒杆的亚硝酸盐清除能力和亚硝胺抑制能力最高,而白花最差。蒸汽烫漂比水浴烫漂能更好的保持黄花菜的抑制亚硝化反应的能力,但均比未烫漂的低。冷冻干燥能更好的保持黄花菜的抗亚硝化活性,日晒干燥次之,热风干燥最差。由此可见,黄花菜提取物对亚硝化反应具有一定的抑制能力,品种不同、烫漂、干燥、冷藏处理均会影响该能力。

Fresh daylily flowers were processed into powder by the following procedures of blanching, drying and comminution, and the powder was extracted with different solvents. The suppression effects of daylily flower exwacts on nitrosation were evaluated in terms of the nitrite scavenging activity and nitrosamine synthesis inhibiting activity detelmined by spectmphotometric method. The 70% methanol extract of daylily flower obtained by the water bath extraction for 0.5 h in an ultrasonic filed had high nitrite scavenging activity and nitrosamine synthesis inhibiting activity, reaching 66.9% and 54.7%, respectively. The nitrite scavenging activity and nitrosamine synthesis inhibiting activity of daylfly flower varied slightly during fridge storage. Among four cultivars （Mengzihua, Baihua, Xiye and Daogan） of daylily flowers, Daogan had the highest nitrite scavenging activity and nitrosamine synthesis inhibiting activity and Xiye the lowest. Stream blanching treatment could maintain higher nitrosamine synthesis inhibiting activity than boiling water blanching treatment, although daylily flowers treated by them both had lower nitrosamine synthesis inhibiting activity than unblanched ones. A highest nitrosamine synthesis inhibiting activity was found in fleeze-dried daylily flowers in comparison with sunlight-dried ones followed by hot air-dried ones. In general daylily flowers possess some nitrosamine synthesis inhibiting activity which is affected by cultivars and blanching, drying and cool storage treatments.