稻米中PCR抑制因子抑制机理的研究

Inhibition Mechanisms of PCR Inhibitors in Rice

针对在检测过程中发现的大米中存在PCR抑制现象,对PCR抑制因子作用机理进行研究。通过把糙米加工成精米和米麸后的实验证明,PCR抑制因子主要存在于糙米的米麸中。研究间接证明PCR抑制因子主要通过抑制Taq聚合酶的酶活来抑制PCR反应。尝试采用多种方法来对大米中的PCR抑制因子进行鉴定,通过非变性电泳(PAGE)和电泳迁移率实验(EMSA)实验排除了大米中的PCR抑制因子是大分子物质。

PCR inhibitory substances in the genornic DNA extracted from brown rice, polished rice and rice bran were analyzed and the inhibition mechanisms were explored. PCR inhibitors were mainly observed in rice bran. Indirect evidence also proved that PCR reaction was inhibited via the inhibition of Taq polymerase. PAGE and EMSA analysis revealed that macromolecules may not be the PCR inhibitors in rice.