紫外诱变赤灵芝原生质体选育高产有机锗菌株的研究

UV Mutagenization of Protoplast for Screening and Breeding of High-yield Organogemanium-producing Strains of Ganoderma lucidum

对赤灵芝原生质体制备、再生及紫外诱变育种、筛选有机锗高产菌株进行研究。结果表明:赤灵芝原生质体制备与再生的最佳条件为菌龄96h,复合酶液浓度为2%溶壁酶、1%蜗牛酶和1%纤维素酶,温度31℃,时间2h,获得赤灵芝原生质体产量为4.33×106个/ml,再生率达0.00275%。原生质体最佳紫外诱变条件为:15W紫外灯,距离30cm,照射30s,致死率为66.7%。最终选育出3株高产有机锗诱变株,有机锗含量分别比出发菌株提高了113.58%、105.87%、103.86%。

Preparation, regeneration, UV mutagenization of Ganoderma lucidum protoplast were studied to obtain high-yield strains producing organogemanium. Under optimized conditions for the preparation and regeneration of Ganoderma lucidum protoplast including cell age 96 h, lywallzyme concentration 2%, glusulase concentration 1%, cellulase concentration 1%, and temperature 31 ~Cfor an enzymolysis duration of 2 h, the protoplast yield and regeneration rate were found to be 4.33 × 10^6 protoplasts/ml and 0.00275%, respectively. When Ganoderma lucidum protoplast was irradiated for 30 s with 15 W ultraviolet lamp 30 cm away from it, the lethality was 66.7% and three high-yield strains were obtained after two passages, the organogemanium contents in whose dried mycelia were higher than that of starting strain by 113.58%, 105.87% and 103.86% separately.