基因PimM在金褐链霉菌SYAU0709中的表达

Expression of PimM Gene in Streptomyces aureofuscus SYAU0709

将纳他霉素生物合成基因簇中的基因PimM置于PermE之下进行整合性表达,克隆至大肠杆菌-链霉菌穿梭质粒载体pSET152中,构建表达载体PBJPIM,将PBJPIM接合、转移、转化金褐霉素产生菌——金褐链霉菌SYAU0709,通过摇瓶发酵和HPLC检测分析发现,有些转化子的金褐霉素产量比野生型菌株提高3～4倍,说明PimM基因在金褐链霉菌中有促进金褐霉素合成的作用,并且遗传性能稳定。

The high-copy-number plasmid PBJPIM, containing the S.aureofuscus PimM gene under the promoter PermE, was constructed. This fragment was ligated with pSET152, resulting in plasmid PBJPIM, and the plasmid PBIPIM was introduced into wild-type S.aureofuscus SYAU0709 via E.coli --Streptomyces aureofuscus conjugation. The aureofuscin yield was found to be increased by 3 - 4 times in some transformants when compared with that in the strain SYAU0709. Our study demonstrates that PimM gene can promote aureofuscin biosynthesis and the recombinant strains obtained have highly stable heritage.