茶氨酸改善小鼠睡眠状况的实验研究

Sleep-promoting Effect of L-Theanine on Mice

以0、50、100、200mg/kg bw·d的茶氨酸剂量灌胃小鼠,30d后进行睡眠功能性评价实验。结果表明,茶氨酸具有改善睡眠的功效,中剂量组(100mg/kg bw·d)的各项功能性评价效果最佳。茶氨酸没有直接的催眠作用,但能有效延长戊巴比妥钠催眠条件下小鼠的睡眠时间[中剂量组的睡眠时间为(40.3±26.0)min],提高巴比妥钠阈下剂量催眠条件下小鼠的入睡率(中剂量组的入睡率达33.3%)以及有效缩短巴比妥钠催眠条件下小鼠的睡眠潜伏期[中剂量组的睡眠潜伏期为(30.32±4.8)min],与空白组相比差异显著。

The effects of L-theanine on barbiturate-induced sleeping time, subthreshold barbiturate-induced sleeping time and barbiturate-induced sleeping latency period in mice were investigate. Healthy ICR mice were randomly divided into four groups of 12 mice each： control group fed with distilled water and three treatment groups fed L-theanine at low-, medium-, and highdoses of 50,100 and 200 mg/（kg bw od）. for 30 days. L-Theanine was able to improve sleep function of mice to a certain extent and the best effect was found in the medium-dose-treated mice. However, no direct hypnotic effect of L-theanine was observed while it was able to prolong the sleeping time induced by pentobarbital sodium [that of the medium-dose-treated mice reached （40.3 ± 26.0） mini and to enhance the sleeping rate induced by barbital sodium at the threshold dose （that of the medium-dose- treated mice reached 33.3%）. L-Theanine also could significantly decrease （P 〈 0.05） the sleeping latency period induced by barbital sodium [that of the medium-dose-treated mice reached （30.32 ± 4.8） mini.