摘要
目的探讨新生儿全身炎症反应综合征(SIRS)时,髓系细胞触发受体1(Triggering Receptor Expressed onMyeloid cells-1,TREM-1)在外周血单核细胞核酸和蛋白质水平的表达及其意义。方法实验对象分为正常对照组,SIRS组,严重SIRS组,SIRS伴休克组。实验组病例确诊后各抽取外周血5ml,按照Dapino P的方法分离单核细胞。四组分别提取RNA及总蛋白。用逆转录聚合酶链反应(RT-PCR)检测TREM-1 mRNA的表达,用Western Blot方法检测TREM-1蛋白的表达。结果各组Trem-1 mRNA/β-actin及TREM-1蛋白/GAPDH比值依次增加,组间差异有统计学意义(P<0.01)。结论新生儿全身炎症反应综合征时TREM-1的表达有明显变化,并随着病情分级严重程度而表达上调。
Objective ： To investigate the expression of triggering reeeptor expressed on myeloid cells - 1 （ TREM - 1 ） in new born infants with systemic inflammatory response syndrome （SIRS）. Methods： Peripheral blood of 33 new born infants with SIRS and 10 healthy volunteers were collected. They were divided into groups A, B, C and D. The expression of TREM - 1 mRNA was detected by semi - quantitative reverse transcriptional polymerase chain reaction （ RT - PCR）. The expression of TREM - 1 protein was detected by Western Blot. Results ： The results showed that the expressions of TREM - 1 mRNA and TREM - 1 proteins in groups A, B, C and D were significantly different among the groups （ P 〈 0.05 ）. Conclusion： The expression of triggering receptor expressed on myeloid cells - 1 （ TREM - 1 ） with systemic inflammatory response syndrome changed obviously, and was up - regulated with the scale of pathogenetic condition.
出处
《中国优生与遗传杂志》
2009年第8期13-14,56,共3页
Chinese Journal of Birth Health & Heredity
