寒凝血瘀型原发性痛经与MHC—DRB1~*基因的相关研究

MHC—DRB1~* gene polymorphism in patients with Primary Dysmenorrhea

目的探讨北方地区汉族人寒凝血瘀型原发性痛经(PD)与人类主要组织相容性复合体MHC—DRB1*基因多态性的关系。方法应用顺序特异性引物和聚合酶链反应(PCR-SSP)技术,对23例寒凝血瘀型原发性痛经患者和32例无血缘关系的健康人MHC—DRB1*各等位基因进行了检测分析,并将该方法与其它检测MHC等位基因的方法进行对比。结果结果表明,MHC—DRB1*09(RR=3.462,P<0.05)基因与PD正相关,显著高于对照组,两组之间比较差异有显著性意义,而其它MHC—DRB1*各等位基因未见异常,均无统计学意义。结论本项研究结果提示,MHC—DRB1*09基因可能是我国北方汉族人寒凝血瘀型PD致病的易感基因,为揭示寒凝血瘀型PD的发病机制中免疫遗传学作用提供了重要信息和依据。

Objective： In order to study the correlativity between MHC-DRB1＊ gene polymorphism and Primary Dysmenorrhea （PD）. Methods： MHC-DRB1＊ gene Polymorphism in 23 patients with PD and 32 normal control subjects was analyzed by using the polymerease chain reaction ／ sequence specific primer （ PCR ／ SSP） technique. 32 healthy people that not related by blood as controlled group, they were examined by the same method. Results ： MHC-DRB1＊ 09 （ RR = 3. 462, P 〈 0. 05）. The frequency of HLA - A＊ 09 allele was higher than controlled group remarkably, the difference between two groups was significant in statistics, the others allele of MHC - DRB1 ＊ were no abnormality seen, there was no difference in statistics. Conclusions ： These findings further verify that immunogenetics play a predominant Pathogenetics role in a subset of PD patients. Individuals carrying HLA - A ＊ 09 alleles may be susceptibility gene to PD. This method is more specific, sensitive, simple, rapid and accurate. It could be applied to the routine clinical detective.