FQ-PCR血、尿、乳汁联合检测在小儿人巨细胞病毒感染中的临床研究

Clinical study of application fluorescent quantitative PCR on diagnosis of HCMV infection in children

目的探讨用荧光定量聚合酶链反应(fluorescence quantitative PCR,FQ-PCR)法进行血、尿、母乳联合检测小儿人巨细胞病毒(HCMV)的临床意义。方法采用FQ-PCR法联合检测182例疑诊HCMV感染的患儿,对母乳喂养的阳性患儿补测母乳HCMV-DNA。结果血、尿及联合检测HCMV-DNA阳性率分别为15.93%、29.12%、39.01%。阳性患儿配对的母乳HCMV-DNA阳性率为45.00%,阴性患儿配对的母乳HCMV-DNA阳性率为18.60%,两组进行χ2检验,χ2=5.54,具有显著性差异(P<0.02)。结论联合检测能提高HCMV-DNA检测阳性率,尿HCMV-DNA检测阳性率高于血HCMV-DNA检测,HCMV感染母乳是婴儿获得性感染的主要途径之一。

Objective： To understand the clinical significance of HCMV combined testing of serum, urine, breast milk samples using fluorescent quantitative polymerase chain reaction （FQ -PCR） in children. Methods： HCMV -DNA in serum and urine was detected by using FQ -PCR in 182 suspect cases, as well as breast milk from mothers, whose children was feed by breast and its HCMV infection was confirmed. Results： The positive rate of HCMV -DNA of serum, urine and combined testing was 15. 93%, 29. 12%, 39. 01%, respectively. The HCMV-DNA positive rate of breast milk from confirmed case＇s mother was significant higher than that of control group （X^2 = 5.54, P 〈 0. 02）. Conclusion： The results indicated that combined testing could increase the positive rate of HCMV - DNA, the HCMV - DNA positive rate of urine was higher than serum, and breast milk infected with HCMV should be the main route of acquired infection.