原发癌及骨转移瘤患者外周血淋巴细胞姐妹染色单体交换频率及微核率的研究

The study of SCE and MN rate of peripheral lymphocytes in the patients with primary cancer and bone metastasis tumor

目的通过对原发癌及骨转移瘤患者外周血淋巴细胞姐妹染色单体交换(sister chromatid exchanges,SCE)率和微核(micronuclei,MN)率的研究,来探讨细胞癌变中DNA的变化。方法检测正常人、原发癌和骨转移癌的外周血淋巴细胞姐妹染色单体交换率和微核率,并比较结果。结果有原发肿瘤的患者姐妹染色单体交换率和微核率增高,而骨转移瘤患者的姐妹染色单体交换率和微核率与无转移瘤患者比较,两者存在显著差异。结论骨转移瘤患者的姐妹染色单体交换率和微核水平损伤严重,姐妹染色单体交换率和微核率能敏感反映DNA的损伤情况,故此姐妹染色单体交换率和微核率检测被用作快速检测环境中诱变、致癌因素的灵敏方法之一。

Objective： To investigate the change of DNA during the process of canceration. Methods： Detect sister chromatid exchanges （SCE） rate and micronuclei （MN） rate in peripheral lymphocytes which are from the patients with primary cancer and the patiences with primary cancer and bone metastasis tumoe. Results： SCE rate and MN rate, which increased among the patients with primary cancer, are significantly different between the patients with primary cancer and the patients with primary cancer and bone metastasis tumor. Conclusion ： The daminifieation of SCE and MN among the patients with primary cancer and bone metastasis was severer than that among the patients with primary cancer. SCE and MN are used to detect those factors which can lead to cancer because they can infect the degree of DNA damage insensitively.