EYA2在非小细胞肺癌中的表达

Expression of EYA2 in non-small cell lung cancer

目的探讨EYA2在非小细胞肺癌（NSCLC）中的表达及其与临床参数之间的关系。方法59例NSCLC组织及癌旁正常肺组织，分别行Western blot分析及免疫组织化学染色，比较癌组织与肺组织之间EYA2表达差异，分析EYA2表达与肺癌临床参数之间的关系。结果EYA2在NSCLC组织中的表达水平升高。EYA2分布于NSCLC细胞浆及细胞核中，胞浆中表达更明显。EYA2的表达与NSCLC病理类型有关，与分化程度、TNM分期、淋巴结转移等因素无关。EYA2在肺腺癌中表达明显升高，而在鳞癌中无变化。结论EYA2在肺腺癌中表达水平升高，可能作为转录激活因子参与肺腺癌的发生、发展。

Objective To identify the expression of Drosophila Eyes Absent Homologue 2 （EYA2） in non-small cell lung cancer （ NSCLC ） and to investigate its correlation with clinical parameters. Methods 59 fresh specimens of lung cancer and paired normal lung tissue were obtained from 59 NSCLC cases treated in the department of thoracic surgery in our hospital from June 2006 to October 2007. Western blotting and immunohistochemistry were used to assay the specimens with goat anti-human EYA2 polyclone antibody. Clinicopathological parameters were collected and the correlation with EYA2 expression was subsequently analyzed. Results The expression of EYA2 was detected in cytoplasm and nucleus of the cancer cells, but mostly in cytoplasm. Western blotting and immunohistochemistry showed the expression of EYA2 in NSCLC was increased and correlated with pathological type, but not with gender, age, pTNM stage, histological differentiation and lymph node metastasis. EYA2 expression was significantly up-regulated in adenocarcinoma, while not changed in lung squamous cell carcinoma. Conclusion The results of this study suggest that expression of EYA2 in lung adenocarcinoma is augmented. EYA2 is likely participating in the development of lung adenocarcinoma as a transcriptional activator.