雌激素β受体对子宫内膜异位症形成的影响

Effect of estrogen receptor β on the formation of mice endometriosis

目的探讨雌激素β受体(ER-β)对子宫内膜异位症形成的影响。方法采用30只ER-β基因敲除(βERKO)小鼠及22只同源未敲除(βER)小鼠建立子宫内膜异位症模型。将两种小鼠各分为3组:保留卵巢组(βERKO10只、βER8只)、切除卵巢组(βERKO10只、βER7只)、切除卵巢+雌激素组(βERKO10只、βER7只)。术后2周分别取出两种小鼠的子宫内膜异位灶,检测其重量、组织形态、MMP-9的蛋白含量,并进行比较分析。结果术后2周保留卵巢组移植物重量明显高于术前(P<0.05),切除卵巢+雌激素组手术前后移植物重量无明显差异(P>0.05),而切除卵巢组术后移植物重量明显低于术前(P<0.05);但βERKO与βER两种小鼠间移植物重量无明显差异(P>0.05)。βERKO与βER两种小鼠异位病灶的组织形态及各部位的MMP-9表达也无显著差异(P>0.05)。结论ER-β对子宫内膜异位症的形成影响微弱。

Objective To investigate the effect of estrogen receptor β （ER-β） on the formation of mice endometriosis. Methods Endometriosis animal model was established with 30 ERβ knockout （βERKO） mice and 22 ER-β non-knockout （βER） mice. All the mice were divided into three groups： intact group （βERKO 10 and βER 8）, ovariectomy group （βERKO 10 and βER 7） and ovariectomy estradiol treatment group （βERKO 10 and βER 7）. The endometriotic grafts were taken out from all the mice 2 weeks after operation, and the weight, histological structure and the expression of MMP9 in the grafts were analyzed and compared between βERKO and βER mice. Results The endometriotic grafts were significantly heavier 2 weeks after transplantation than that before transplantation in intact group （P〈0. 05）, and were lighter than that before transplantation in ovariectomy group （P〈0. 05）, but no significant difference was found before and 2 weeks after transplantation in ovariectomy ＋ estradiol treatment group （P〉0. 05）. In addition, no significant differences were found between the βERKO and the βER mice in endometriotic grafts＇ weight, the histopathologic structure and the expression of MMP-9. Conclusion ER may throw few influence on the formation of endometriosis.