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鸡Mx基因的克隆与原核表达 被引量：2

Cloning and Expression of Chicken Mx cDNA in Escherichia coli
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摘要 本研究旨在通过克隆鸡Mx全基因序列进而进行该基因的原核表达,获得具有生物学活性的蛋白。利用poly I:C诱导鸡胚成纤维细胞Mx基因表达,克隆了Mx基因全长cDNA序列,将开放阅读框(ORF)连接构建于表达质粒pGEX-4t-2中获得重组表达载体pGEX-Mx,转化Rosetta(DE3)菌株,经IPTG诱导后检测。表达产物检测显示该蛋白的相对分子量为75 ku。说明获得了Mx基因的高效表达,为进一步进行Mx基因的活性检测以及利用Mx蛋白进行抗病毒转基因的研究奠定了基础。 This experiment was conducted to study Mx gene expression in E. coli under the condition of cloning Mx gene complete sequence. Chicken embryo fibroblast （CEF） were treated with poly I：C to stimulate Mx gene expression. Full length cDNA of Mx was cloned. Recombinant expression vector pGEX-Mx including Mx ORF was constructed using pGEX-4t-2 expression system to transfer Escherichia coli Rosetta （DE3） strain. The result showed that the relative molecular weight of expressed protein was about 75 ku after induction with IPTG, suggesting that Mx gene has been efficiently expressed. The research laid a good foundation for further studying on bioactivity assay, exploring new way of antiviral medication and transgenic study.
作者 尹春光 杜立新 李善刚 魏彩虹 刘涛 李宏滨 赵桂平
机构地区 中国农业科学院北京畜牧兽医研究所 山东省济宁学院
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2009年第7期978-981,共4页 ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金 北京市自然科学基金资助项目(5051004) 国家高技术研究发展计划(863计划)项目(2007AA10Z183)
关键词 鸡 MX蛋白 克隆 表达 chicken Mx protein cloning expression
分类号 S831.2 [农业科学—畜牧学] Q344.13 [生物学—遗传学]
  • 相关文献

参考文献11

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共引文献6

同被引文献14

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畜牧兽医学报
2009年 第7期

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