鹅IDH1基因的分离、序列分析及表达特征研究

Isolation,Sequence Analysis and Expression Profile of IDH1 in Goose Liver

为阐明鹅肥肝形成的机制,使用mRNA差异显示技术研究朗德鹅和溆浦鹅在超饲养和正常饲养条件下肝脏基因表达差异。基因IDH1被证实在2个品种鹅肝脏中表达受到显著抑制(P<0.05)。结果,得到了该基因1269 bp的CDS序列,与鸡IDH1有95%的同源性。序列分析表明,该序列含有1个1248 bp的开放读码框(ORF),编码含415个氨基酸的蛋白质,该蛋白质序列存在1个保守结构域Icd,与其它物种该蛋白的同源性分别为:鸡99%、大鼠89%、人90%、猿90%、牛88%、小鼠88%。应用生物信息学的方法对该蛋白质的功能和结构进行了分析。组织表达分析表明,鹅IDH1基因在肝脏中表达丰富,在脾、肾和肌胃中中等表达,在其它组织中表达量较低。研究结果显示,超饲可以抑制鹅肝脏IDH1基因mRNA的表达。

To obtain an understanding of fattening mechanism of fatty liver, mRNA differential display reverse transcription PCR （DDRT-PCR） was applied to study the differences of gene expression in French Landes Grey goose and Xupu White goose in different conditions of overfeeding and normal feeding. One gene was found to be significantly down-regulated in fatty liver in both breeds（P%0.05）, which has a CDS length of 1 269 bp and 95% identity to chicken IDH1. The sequence analysis revealed that its 1 248 bp-in-length open reading frame （ORF） encodes a 415-amino-acids protein containning a putative conserved domain of Icd and has high homology with chieken（99%）, rat（89%）, human（90%）, monkey（90%）, cattle（88M） ,and mouse（88%）, respectively. The structure and function of this protein were analyzed using bioinformaties methods. The tissue expression analysis indicated that goose IDH1 mRNA was higher expressed in liver than that in other tested tissues. The present results suggested that overfeeding could decrease the mRNA expression level of goose IDH1.